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Anti-HSPA12A Polyclonal Antibody
Catalog No. TMAB-07353 Copy Product Info
🥰Excellent
Anti-HSPA12A Polyclonal Antibody is a Rabbit antibody targeting HSPA12A. Anti-HSPA12A Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-HSPA12A Polyclonal Antibody
Copy Product Info🥰Excellent
Catalog No. TMAB-07353
Anti-HSPA12A Polyclonal Antibody is a Rabbit antibody targeting HSPA12A. Anti-HSPA12A Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-HSPA12A Polyclonal Antibody
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 50 μL | $222 | 7-10 days | 7-10 days | |
| 100 μL | $373 | 7-10 days | 7-10 days | |
| 200 μL | $528 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
Stability & Storage
| Description | Anti-HSPA12A Polyclonal Antibody is a Rabbit antibody targeting HSPA12A. Anti-HSPA12A Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Reactivity | Human,Mouse (predicted:Rat,Chicken,Dog,Pig,Cow,Horse,Rabbit,Sheep) |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (HSPA12A) Polyclonal Antibody, Unconjugated (TMAB-07353) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation: Anti-HSPA12A Antibody, conjugated (TMAB-07353-FITC) 1:400, 1.5 hours at 37°C; DAPI (5 μg/ml, blue) was used to stain the cell nuclei. 3. Sample: A673 Cell (Human) Lysate at 40 μg Primary: Anti-HSPA12A (TMAB-07353) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 75 kD Observed band size: 73 kD |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:200-800 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Tissue Specificity | Widely expressed with highest levels in brain, kidney and muscle. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | The heat shock proteins (HSPs) comprise a group of highly conserved, abundantly expressed proteins with diverse functions, including the assembly and sequestering of multi-protein complexes, the transportation of nascent polypeptide chains across cellular membranes and the regulation of protein folding. HSPA12A (heat shock 70kDa protein 12A) is a 675 amino acid protein that belongs to the HSP 70 family and is expressed in muscle, kidney and brain where it may play a role in cellular metabolism and regulation of neuronal function. The gene encoding HSPA12A maps to human chromosome 10, which houses over 1,200 genes and comprises nearly 4.5% of the human genome. Defects in some of the genes that map to chromosome 10 are associated with Charcot-Marie Tooth disease, Jackson-Weiss syndrome, Usher syndrome, nonsyndromatic deafness, Wolman’s syndrome, Cowden syndrome, multiple endocrine neoplasia type 2 and porphyria. |
| Immunogen | KLH conjugated synthetic peptide: human HSPA12A |
| Antigen Species | Human |
| Gene Name | HSPA12A |
| Gene ID | |
| Protein Name | Heat shock 70 kDa protein 12A |
| Uniprot ID |
| Molecular Weight | Theoretical: 75 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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