Anti-HSPA12A Antibody (5N278) is a Rabbit antibody targeting HSPA12A. Anti-HSPA12A Antibody (5N278) can be used in ICC/IF, IF, IHC-Fr, IHC-P, IP, WB.

IgG

5N278

Human,Mouse,Rat

1. 25 ug total protein per lane of various lysates (see on figure) probed with HSPA12A monoclonal antibody, unconjugated (TMAB-07354) at 1: 1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
2. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with HSPA12A Monoclonal Antibody, Unconjugated (TMAB-07354) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with HSPA12A Monoclonal Antibody, Unconjugated (TMAB-07354) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with HSPA12A Monoclonal Antibody, Unconjugated (TMAB-07354) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with HSPA12A Monoclonal Antibody, Unconjugated (TMAB-07354) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.

ICC/IF=1:50-200; IF=1:100-500; IHC-Fr=1:100-500; IHC-P=1:100-500; IP=1:20-50; WB=1:500-2000

Monoclonal

A synthesized peptide: human Hsp70 12A

Human

HSPA12A

Heat shock 70 kDa protein 12A