Anti-Hsp90 alpha Antibody (9E708) is a Rabbit antibody targeting Hsp90 alpha. Anti-Hsp90 alpha Antibody (9E708) can be used in FCM, ICC/IF, IF, IHC-Fr, IHC-P, WB.

IgG

9E708

Human,Mouse,Rat

1. 25 ug total protein per lane of various lysates (see on figure) probed with Hsp90 alpha monoclonal antibody, unconjugated (TMAB-07347) at 1:2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
2. Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Hsp90 alpha Monoclonal Antibody, Unconjugated (TMAB-07347) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Hsp90 alpha Monoclonal Antibody, Unconjugated (TMAB-07347) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Hsp90 alpha Monoclonal Antibody, Unconjugated (TMAB-07347) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Hsp90 alpha Monoclonal Antibody, Unconjugated (TMAB-07347) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Hsp90 alpha Monoclonal Antibody, Unconjugated (TMAB-07347) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
7. Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Hsp90 alpha Monoclonal Antibody, Unconjugated (TMAB-07347) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
8. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Hsp90 alpha Monoclonal Antibody, Unconjugated (TMAB-07347) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
9. Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Hsp90 alpha Monoclonal Antibody, Unconjugated (TMAB-07347) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
10. Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Hsp90 alpha Monoclonal Antibody, Unconjugated (TMAB-07347) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
11. Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Hsp90 alpha Monoclonal Antibody, Unconjugated (TMAB-07347) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
12. Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Hsp90 alpha Monoclonal Antibody, Unconjugated (TMAB-07347) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
13. Paraformaldehyde-fixed, paraffin embedded Mouse Breast; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Hsp90 alpha Monoclonal Antibody, Unconjugated (TMAB-07347) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
14. Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Hsp90 alpha Monoclonal Antibody, Unconjugated (TMAB-07347) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
15. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Hsp90 alpha Monoclonal Antibody, Unconjugated (TMAB-07347) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
16. Paraformaldehyde-fixed, paraffin embedded Human Prostate Tumor; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Hsp90 alpha Monoclonal Antibody, Unconjugated (TMAB-07347) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining
17. 4% Paraformaldehyde-fixed K562 (H) cell; Triton X-100 at r. T. for 20 min; Antibody incubation with (Hsp90 alpha) monoclonal Antibody, unconjugated (TMAB-07347) 1: 100, 90 min at 37°C; followed by BF488 conjugated Goat Anti-Rabbit IgG antibody (green, BF488) at 37°C for 90 min, DAPI (blue) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
18. 4% Paraformaldehyde-fixed Jurkat (H) cell; Triton X-100 at r. T. for 20 min; Antibody incubation with (Hsp90 alpha) monoclonal Antibody, unconjugated (TMAB-07347) 1: 100, 90 min at 37°C; followed by BF488 conjugated Goat Anti-Rabbit IgG antibody (green, BF488) at 37°C for 90 min, DAPI (blue) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
19. 4% Paraformaldehyde-fixed K562 (H) cell; Triton X-100 at r. T. for 20 min; Antibody incubation with (Hsp90 alpha) monoclonal Antibody, unconjugated (TMAB-07347) 1: 100, 90 min at 37°C; followed by BF488 conjugated Goat Anti-Rabbit IgG antibody (green, BF488) at 37°C for 90 min, DAPI (blue) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
20. The K562 (H) cells were fixed with 4% PFA (10 min at r. T.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-Hsp90 alpha antibody (TMAB-07347, 1: 100); Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed.
21. The Jurkat (H) cells were fixed with 4% PFA (10 min at r. T.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-Hsp90 alpha antibody (TMAB-07347, 1: 100); Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed.

FCM=1:50-100; ICC/IF=1:50-200; IF=1:200-500; IHC-Fr=1:200-500; IHC-P=1:200-500; WB=1:1000-5000

Monoclonal

A synthesized peptide: human Hsp90 alpha

Human

HSP90AA1

Heat shock protein HSP 90-alpha

Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function.