Anti-HSP90 alpha Antibody (4L975) is a Mouse antibody targeting HSP90 alpha. Anti-HSP90 alpha Antibody (4L975) can be used in WB,IHC-P,IHC-Fr,IF.

IgG

4L975

Human,Mouse,Rat

1. Paraformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (HSP90 alpha) Monoclonal Antibody, Unconjugated (TMAB-07346) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded (Human colon cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (HSP90 alpha) Monoclonal Antibody, Unconjugated (TMAB-07346) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.
3. Sample:
Lane 1: Mouse Testis tissue lysates
Lane 2: Mouse Cerebrum tissue lysates
Lane 3: Mouse Placenta tissue lysates
Lane 4: Rat Placenta tissue lysates
Lane 5: Human U251 cell lysates
Lane 6: Human Molt-4 cell lysates
Lane 7: Human HL60 cell lysates
Lane 8: Human A549 cell lysates
Primary: Anti-HSP90 alpha (TMAB-07346) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 81 kD
Observed band size: 81 kD
4. Sample:
Hela Cell (Human) Lysate at 40 μg
NIH/3T3 Cell (Mouse) Lysate at 40 μg
Primary: Anti-HSP90 alpha (TMAB-07346) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 81 kD
Observed band size: 98 kD
5. Sample:
293T (Human) Cell Lysate at 30 μg
NIH/3T3 (Mouse) Cell Lysate at 40 μg
Primary: Anti-HSP90 alphA (TMAB-07346) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 81 kD
Observed band size: 81 kD
6. Paraformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (HSP90 alpha) Monoclonal Antibody, Unconjugated (ascites of TMAB-07346 3H7) at 1: 2000 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

WB: 1:500-1000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Monoclonal

Recombinant Protein: human HSP90 alpha Protein

Human

HSP90AA1

Heat shock protein HSP 90-alpha

Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function.