Anti-HSP70 Antibody (5U19) is a Mouse antibody targeting HSP70. Anti-HSP70 Antibody (5U19) can be used in WB,IHC-P,IHC-Fr,IF.

IgG

5U19

Human,Mouse,Rat

1. Paraformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (HSP70) Monoclonal Antibody, Unconjugated (TMAB-07345) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
2. Sample: Hela Cell (Human) Lysate at 40 μg
Primary: Anti-HSP70 (TMAB-07345) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 70 kD
Observed band size: 70 kD
3. Sample: HepG2 Cell (Human) Lysate at 40 μg
Primary: Anti-HSP70 (TMAB-07345) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 70 kD
Observed band size: 70 kD
4. Paraformaldehyde-fixed, paraffin embedded (Rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (HSP70) Monoclonal Antibody, Unconjugated (ascites of TMAB-07345 5G10) at 1: 2000 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded (Human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (HSP70) Monoclonal Antibody, Unconjugated (ascites of TMAB-07345 5G10) at 1: 2000 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (HSP70) Monoclonal Antibody, Unconjugated (ascites of TMAB-07345 5G10) at 1: 2000 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

WB: 1:500-1000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Monoclonal

KLH conjugated synthetic peptide: human Hsp70

Human

HSPA1A

Heat shock 70 kDa protein 1A

In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. In case of rotavirus A infection, serves as a post-attachment receptor for the virus to facilitate entry into the cell.