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Anti-HSP70 Antibody (3B173)
😃Good
Catalog No. TMAY-01968
Alias heat shock 70kDa protein 1A
Anti-HSP70 Antibody (3B173) is a Mouse antibody targeting HSP70. Anti-HSP70 Antibody (3B173) can be used in WB,IP.
Anti-HSP70 Antibody (3B173)
Anti-HSP70 Antibody (3B173)
😃Good
Catalog No. TMAY-01968Alias heat shock 70kDa protein 1A
Anti-HSP70 Antibody (3B173) is a Mouse antibody targeting HSP70. Anti-HSP70 Antibody (3B173) can be used in WB,IP.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 100 μL | $199 | 7-10 days |
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Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Anti-HSP70 Antibody (3B173) is a Mouse antibody targeting HSP70. Anti-HSP70 Antibody (3B173) can be used in WB,IP. |
| Alias | heat shock 70kDa protein 1A |
| Ig Type | Monoclonal Mouse IgG1 |
| Clone | 3B173 |
| Reactivity | Human |
| Verified Activity | 1. Anti-HSPA1A mouse monoclonal antibody at 1:500 dilution. -Lane A: A431 Whole Cell Lysate. -Lane B: A549 Whole Cell Lysate. -Lane C: HepG2 Whole Cell Lysate. -Lane D: Jurkat Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Mouse IgG (H+L)/HRP at 1/10000 dilution. -Developed using the ECL technique. -Performed under reducing conditions. -Predicted band size:70 kDa. -Observed band size:71 kDa. 2. HSPA1A was immunoprecipitated using: -Lane A:0.5 mg HepG2 Whole Cell Lysate. -4 µL anti-HSPA1A mouse monoclonal antibody and 60 μg of Immunomagnetic beads Protein A/G. -Primary antibody: -Anti-HSPA1A mouse monoclonal antibody, at 1:100 dilution. -Secondary antibody: -Rabbit Anti-Mouse IgG F(ab)2/HRP at 1/10000 dilution. -Developed using the ECL technique. -Performed under reducing conditions. -Predicted band size: 70 kDa. -Observed band size:70 kDa |
| Application | WB,IP |
| Recommended Dose | WB: 1:500-1:2000; IP: 1-5 µL/mg of lysate |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Construction | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human HSP70 (rh HSP70; TMPY-02443; NP_005337.2; Ala2-Asp641). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | HSPA1A is a member of the Hsp70 protein family. The 70 kilodalton heat shock proteins (Hsp70s) are a family of ubiquitously expressed heat shock proteins. HSP are abundant and conserved proteins present in all cells. Upon temperature shock or other stress stimuli, HSP is synthesized intracellularly, which may protect cells from protein denaturation or death. Extracellularly, HSP can serve a cytokine function to initiate both innate and adaptive immunity through activation of APC. HSP serves also a chaperone function and facilitates the presentation of antigen peptide to T cells. Molecular chaperones of the Hsp70 family have diverse functions in cells. They assist the folding of newly synthesized and stress-denatured proteins, as well as the import of proteins into organelles, and the dissociation of aggregated proteins. The well-conserved Hsp70 chaperones are ATP dependent: binding and hydrolysis of ATP regulate their interactions with unfolded polypeptide substrates, and ATPase cycling is necessary for their function. All cellular functions of Hsp70 chaperones use the same mechanism of ATP-driven polypeptide binding and release. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Human HSP70 Protein (TMPY-02443) |
| Antigen Species | Human |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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