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Anti-HSP60 Polyclonal Antibody

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Catalog No. TMAB-00892
Alias Hsp60, heat shock 60kDa protein 1 (chaperonin), 60kDa

Anti-HSP60 Polyclonal Antibody is a Rabbit antibody targeting HSP60. Anti-HSP60 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.

Anti-HSP60 Polyclonal Antibody

Anti-HSP60 Polyclonal Antibody

😃Good
Catalog No. TMAB-00892Alias Hsp60, heat shock 60kDa protein 1 (chaperonin), 60kDa
Anti-HSP60 Polyclonal Antibody is a Rabbit antibody targeting HSP60. Anti-HSP60 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.
Pack SizePriceAvailabilityQuantity
50 μL$2217-10 days
100 μL$3747-10 days
200 μL$5277-10 days
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Product Introduction

Bioactivity
Description
Anti-HSP60 Polyclonal Antibody is a Rabbit antibody targeting HSP60. Anti-HSP60 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.
AliasHsp60, heat shock 60kDa protein 1 (chaperonin), 60kDa
Ig Type
IgG
Reactivity
Human,Mouse,Rat (predicted:Dog,Cow,Horse,Rabbit)
Verified Activity
1. Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-HSP60 Polyclonal Antibody, Unconjugated (TMAB-00892) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-HSP60 Polyclonal Antibody, Unconjugated (TMAB-00892) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
3. Paraformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (HSP60) Polyclonal Antibody, Unconjugated (TMAB-00892) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining.
4. Sample:
Lane 1: Liver (Mouse) Lysate at 40 μg
Lane 2: Adrenal gland (Mouse) Lysate at 40 μg
Lane 3: NIH/3T3 (Mouse) Cell Lysate at 30 μg
Lane 4: Adrenal gland (Rat) Lysate at 40 μg
Lane 5: Jurkat (Human) Cell Lysate at 30 μg
Lane 6: MDA-MB-231 (Human) Cell Lysate at 30 μg
Lane 7: HepG2 (Human) Cell Lysate at 30 μg
Lane 8: Hela (Human) Cell Lysate at 30 μg
Lane 9: K562 (Human) Cell Lysate at 30 μg
Primary: Anti-HSP60 (TMAB-00892) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 62 kDa
Observed band size: 62 kDa
5. HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (HSP60) polyclonal Antibody, Unconjugated (TMAB-00892) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nucleus.
Application
WB,IHC-P,IHC-Fr,ICC/IF,IF
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:100; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationMitochondrion matrix.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundThis gene encodes a member of the chaperonin family. The encoded mitochondrial protein may function as a signaling molecule in the innate immune system. This protein is essential for the folding and assembly of newly imported proteins in the mitochondria. This gene is adjacent to a related family member and the region between the 2 genes functions as a bidirectional promoter. Two pseudogenes, both located on chromosome 8, have been associated with this gene. Two transcript variants encoding the same protein have been identified for this gene. Mutations associated with this gene cause autosomal recessive spastic paraplegia 13.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human HSP60
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
Signal transduction proteins ELISA kits,Heat Shock Proteins,Mitochondria
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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