Anti-HSP47 Antibody (1C176) is a Rabbit antibody targeting HSP47. Anti-HSP47 Antibody (1C176) can be used in IF, IHC-Fr, IHC-P, WB.

IgG

1C176

Human,Mouse

1. 25 ug total protein per lane of various lysates (see on figure) probed with HSP47 monoclonal antibody, unconjugated (TMAB-07343) at 1: 1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
2. Paraformaldehyde-fixed, paraffin embedded Mouse Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with HSP47 Monoclonal Antibody, Unconjugated (TMAB-07343) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with HSP47 Monoclonal Antibody, Unconjugated (TMAB-07343) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Human Esophagus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with HSP47 Monoclonal Antibody, Unconjugated (TMAB-07343) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded Human Esophageal Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with HSP47 Monoclonal Antibody, Unconjugated (TMAB-07343) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.

IF=1:100-500; IHC-Fr=1:100-500; IHC-P=1:100-500; WB=1:500-2000

Monoclonal

A synthesized peptide: human Serpin H1

Human

SERPINH1

Serpin H1

Binds specifically to collagen. Could be involved as a chaperone in the biosynthetic pathway of collagen.