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Anti-Hsp27/HSPB1 Antibody (8B935)
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Catalog No. TMAY-00671
Alias SRP27, HSPB1, HSP28, Hsp27, HSP 27, HMN2B, CMT2F
Anti-Hsp27/HSPB1 Antibody (8B935) is a Mouse antibody targeting Hsp27/HSPB1. Anti-Hsp27/HSPB1 Antibody (8B935) can be used in FCM,ICC/IF.
Anti-Hsp27/HSPB1 Antibody (8B935)
Anti-Hsp27/HSPB1 Antibody (8B935)
😃Good
Catalog No. TMAY-00671Alias SRP27, HSPB1, HSP28, Hsp27, HSP 27, HMN2B, CMT2F
Anti-Hsp27/HSPB1 Antibody (8B935) is a Mouse antibody targeting Hsp27/HSPB1. Anti-Hsp27/HSPB1 Antibody (8B935) can be used in FCM,ICC/IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 100 μL | $199 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Mouse Monoclonal Application: FCM, ICC/IF Reactivity: Human |
| Alias | SRP27, HSPB1, HSP28, Hsp27, HSP 27, HMN2B, CMT2F |
| Ig Type | Mouse IgG1 |
| Clone | 8B935 |
| Reactivity | Human |
| Verified Activity | 1. Immunofluorescence staining of Human HSP27 in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with mouse anti-Human HSP27 monoclonal antibody (1:60). Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody and countstained with DAPI (blue). Positive staining was localized to cytoplasm. 2. Flow cytometric analysis of Human HSP27 expression on HeLa cells. The cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Human HSP27, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. |
| Application | FCM,ICC/IF |
| Recommended Dose | ICC-IF: 1:20-1:100; FCM: 1:25-1:100 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Construction | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human HSP27 / HSPB1 (rh HSP27 / HSPB1; TMPY-00823; NP_001532.1; Met1-Lys205). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Conjucates | Unconjugated |
| Others Formats | FITC |
| Antibody Types Available | 2 |
| Immunogen | Recombinant Protein: Human HSP27 / HSPB1 protein (TMPY-00823) |
| Antigen Species | Human |
| Biology Area | Cancer Drug Targets |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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