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Anti-Hsp27/HSPB1 Antibody (8B935)

😃Good
Catalog No. TMAY-00671
Alias SRP27, HSPB1, HSP28, Hsp27, HSP 27, HMN2B, CMT2F

Anti-Hsp27/HSPB1 Antibody (8B935) is a Mouse antibody targeting Hsp27/HSPB1. Anti-Hsp27/HSPB1 Antibody (8B935) can be used in FCM,ICC/IF.

Anti-Hsp27/HSPB1 Antibody (8B935)

Anti-Hsp27/HSPB1 Antibody (8B935)

😃Good
Catalog No. TMAY-00671Alias SRP27, HSPB1, HSP28, Hsp27, HSP 27, HMN2B, CMT2F
Anti-Hsp27/HSPB1 Antibody (8B935) is a Mouse antibody targeting Hsp27/HSPB1. Anti-Hsp27/HSPB1 Antibody (8B935) can be used in FCM,ICC/IF.
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100 μL$199 7-10 days
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Product Introduction

Bioactivity
Description
Anti-Hsp27/HSPB1 Antibody (8B935) is a Mouse antibody targeting Hsp27/HSPB1. Anti-Hsp27/HSPB1 Antibody (8B935) can be used in FCM,ICC/IF.
AliasSRP27, HSPB1, HSP28, Hsp27, HSP 27, HMN2B, CMT2F
Ig Type
Monoclonal Mouse IgG1
Clone
8B935
Reactivity
Human
Verified Activity
1. Immunofluorescence staining of Human HSP27 in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with mouse anti-Human HSP27 monoclonal antibody (1:60). Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody and countstained with DAPI (blue). Positive staining was localized to cytoplasm.

2. Flow cytometric analysis of Human HSP27 expression on HeLa cells. The cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Human HSP27, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
Application
FCM,ICC/IF
Recommended Dose
ICC-IF: 1:20-1:100; FCM: 1:25-1:100
Antibody Type
Monoclonal
Host SpeciesMouse
ConstructionThis antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human HSP27 / HSPB1 (rh HSP27 / HSPB1; TMPY-00823; NP_001532.1; Met1-Lys205). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
PurificationProtein A
AppearanceLiquid
Formulation0.2 μm filtered solution in PBS
Related Conjugates and Formulations
Conjucates
Unconjugated
Others Formats
FITC
Antibody Types Available
2
Antigen Details
Immunogen
Recombinant Human HSP27 / HSPB1 protein (TMPY-00823)
Antigen Species
Human
Biology Area
Cancer Drug Targets
Chemical Properties
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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