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Anti-Hsp27/HSPB1 Antibody (5Q970)
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Catalog No. TMAC-02039
Alias SRP27, HSPB1, HSP28, Hsp27, HSP 27, HMN2B, CMT2F
Anti-Hsp27/HSPB1 Antibody (5Q970) is a Rabbit antibody targeting Hsp27/HSPB1. Anti-Hsp27/HSPB1 Antibody (5Q970) can be used in WB,ICC/IF,IHC,IP,FCM.
Anti-Hsp27/HSPB1 Antibody (5Q970)
Anti-Hsp27/HSPB1 Antibody (5Q970)
😃Good
Catalog No. TMAC-02039Alias SRP27, HSPB1, HSP28, Hsp27, HSP 27, HMN2B, CMT2F
Anti-Hsp27/HSPB1 Antibody (5Q970) is a Rabbit antibody targeting Hsp27/HSPB1. Anti-Hsp27/HSPB1 Antibody (5Q970) can be used in WB,ICC/IF,IHC,IP,FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $297 | 7-10 days | |
| 100 μL | $497 | 7-10 days |
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| Description | Anti-Hsp27/HSPB1 Antibody (5Q970) is a Rabbit antibody targeting Hsp27/HSPB1. Anti-Hsp27/HSPB1 Antibody (5Q970) can be used in WB,ICC/IF,IHC,IP,FCM. |
| Synonyms | SRP27, HSPB1, HSP28, Hsp27, HSP 27, HMN2B, CMT2F |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Western blot analysis of Hsp27 on A549 cells lysates using anti-LRP1 antibody at 1/1,000 dilution. 2. Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Hsp27 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Hsp27 antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Hsp27 antibody. Counter stained with hematoxylin. 5. ICC staining Hsp27 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 6. ICC staining Hsp27 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. Flow cytometric analysis of Hela cells with Hsp27 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
| Application | |
| Recommended Dose | WB: 1:1000-2000; IHC: 1:50-200; ICC/IF: 1:100-500; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | The heat shock proteins (HSPs) comprise a group of highly conserved, abundantly expressed proteins with diverse functions, including the assembly and sequestering of multiprotein complexes, transportation of nascent poly-peptide chains across cellular membranes and regulation of protein folding. Heat shock proteins (also known as molecular chaperones) fall into six general families: HSP 90, HSP 70, HSP 60, the low molecular weight HSPs, the immunophilins and the HSP 110 family. The low molecular weight family includes HSP 10, HSP 20, HSP 27, HSP 32 and HSP 40. HSP 27 is a constitutively expressed cytoplasmic protein that co-localizes to the nucleus upon stress induced by insult. Heat, cytokines and hormones are among the factors that stimulate the synthesis of HSP 27. In vitro, HSP 27 becomes highly phosphorylated following exposure to stress. The discovery that HSP 27 is regulated by hormones such as estrogen has led to studies establishing a relationship between HSP 27 and breast cancer. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Uniprot ID |
| Molecular Weight | Theoretical: 27 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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