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Anti-HSP22 Antibody (9O929)
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Catalog No. TMAB-07333
Anti-HSP22 Antibody (9O929) is a Mouse antibody targeting HSP22. Anti-HSP22 Antibody (9O929) can be used in WB,IHC-P,IHC-Fr,IF.
Anti-HSP22 Antibody (9O929)
Anti-HSP22 Antibody (9O929)
Copy Product Info😃Good
Catalog No. TMAB-07333
Anti-HSP22 Antibody (9O929) is a Mouse antibody targeting HSP22. Anti-HSP22 Antibody (9O929) can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 200 μg | $1,058 | 7-10 days | 7-10 days | |
| 50 μL | $221 | 7-10 days | 7-10 days | |
| 100 μL | $374 | 7-10 days | 7-10 days | |
| 200 μL | $528 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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| Description | Anti-HSP22 Antibody (9O929) is a Mouse antibody targeting HSP22. Anti-HSP22 Antibody (9O929) can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Clone | 9O929 |
| Reactivity | Human |
| Verified Activity | 1. Sample: Lane 1: Human HepG2 cell lysates Lane 2: Human MCF-7 cell lysates Lane 3: Human HeLa cell lysates Lane 4: Human 293T cell lysates Lane 5: Human U-2 OS cell lysates Primary: Anti-HSP22 (TMAB-07333) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 22 kDa Observed band size: 26 kDa 2. Paraformaldehyde-fixed, paraffin embedded Human Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with HSP22 Monoclonal Antibody, Unconjugated (TMAB-07333) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining. |
| Application | |
| Recommended Dose | WB: 1:500-1000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Subcellular Localization | Cytoplasm. Nucleus. Note=Translocates to nuclear foci during heat shock. |
| Tissue Specificity | Predominantly expressed in skeletal muscle and heart. |
| Construction | Hybridoma Monoclonal Antibody |
| Purification | Protein G purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | Hsp22 (HSPB8) is a 196-amino acid protein that contains a central portion homologous to a highly conserved HSP-alpha crystallin domain common to all the small heat shock protein (HSP20) family members. Hsp22 is a monomeric protein which interacts with HSPB1. It displays temperature-dependent chaperone activity. The highest abundance of Hsp22 is in skeletal muscle, heart, and placenta. Mutations in the HSPB8 gene are associated with the inherited peripheral neuropathies, autosomal dominant distal hereditary motor neuropathy type IIA (dSMA) and axonal Charcot-Marie-Tooth disease type 2L (CMT2L). |
| Immunogen | Recombinant Protein: human HSP22 Protein |
| Antigen Species | Human |
| Gene Name | HSPB8 |
| Gene ID | |
| Protein Name | Heat shock protein beta-8 |
| Uniprot ID | |
| Function | Displays temperature-dependent chaperone activity. |
| Molecular Weight | Theoretical: 22 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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