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Anti-HSP22 Antibody (9O929)

Catalog No. TMAB-07333 Copy Product Info
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Anti-HSP22 Antibody (9O929) is a Mouse antibody targeting HSP22. Anti-HSP22 Antibody (9O929) can be used in WB,IHC-P,IHC-Fr,IF.

Anti-HSP22 Antibody (9O929)

Copy Product Info
🥰Excellent
Catalog No. TMAB-07333

Anti-HSP22 Antibody (9O929) is a Mouse antibody targeting HSP22. Anti-HSP22 Antibody (9O929) can be used in WB,IHC-P,IHC-Fr,IF.

Anti-HSP22 Antibody
(9O929)
Pack SizePriceUSA StockGlobal StockQuantity
50 μL$2217-10 days7-10 days
100 μL$3747-10 days7-10 days
200 μL$5287-10 days7-10 days
In stock · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-HSP22 Antibody (9O929) is a Mouse antibody targeting HSP22. Anti-HSP22 Antibody (9O929) can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG
Clone
9O929
Reactivity
Human
Verified Activity
1. Sample:
Lane 1: Human HepG2 cell lysates
Lane 2: Human MCF-7 cell lysates
Lane 3: Human HeLa cell lysates
Lane 4: Human 293T cell lysates
Lane 5: Human U-2 OS cell lysates
Primary: Anti-HSP22 (TMAB-07333) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 22 kDa
Observed band size: 26 kDa
2. Paraformaldehyde-fixed, paraffin embedded Human Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with HSP22 Monoclonal Antibody, Unconjugated (TMAB-07333) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.
Application
Recommended Dose
WB: 1:500-1000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Monoclonal
Host SpeciesMouse
Subcellular LocalizationCytoplasm. Nucleus. Note=Translocates to nuclear foci during heat shock.
Tissue SpecificityPredominantly expressed in skeletal muscle and heart.
ConstructionHybridoma Monoclonal Antibody
PurificationProtein G purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundHsp22 (HSPB8) is a 196-amino acid protein that contains a central portion homologous to a highly conserved HSP-alpha crystallin domain common to all the small heat shock protein (HSP20) family members. Hsp22 is a monomeric protein which interacts with HSPB1. It displays temperature-dependent chaperone activity. The highest abundance of Hsp22 is in skeletal muscle, heart, and placenta. Mutations in the HSPB8 gene are associated with the inherited peripheral neuropathies, autosomal dominant distal hereditary motor neuropathy type IIA (dSMA) and axonal Charcot-Marie-Tooth disease type 2L (CMT2L).
Antigen Details
Immunogen
Recombinant Protein: human HSP22 Protein
Antigen Species
Human
Gene Name
HSPB8
Gene ID
Protein Name
Heat shock protein beta-8
Uniprot ID
Function
Displays temperature-dependent chaperone activity.
Chemical Properties
Molecular WeightTheoretical: 22 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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