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Anti-HSD3B1+ HSD3B2 Polyclonal Antibody
Catalog No. TMAB-07323 Copy Product Info
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Anti-HSD3B1+ HSD3B2 Polyclonal Antibody is a Rabbit antibody targeting HSD3B1+ HSD3B2. Anti-HSD3B1+ HSD3B2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-HSD3B1+ HSD3B2 Polyclonal Antibody
Copy Product Info🥰Excellent
Catalog No. TMAB-07323
Anti-HSD3B1+ HSD3B2 Polyclonal Antibody is a Rabbit antibody targeting HSD3B1+ HSD3B2. Anti-HSD3B1+ HSD3B2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-HSD3B1+ HSD3B2 Polyclonal Antibody
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 50 μL | $220 | 7-10 days | 7-10 days | |
| 100 μL | $374 | 7-10 days | 7-10 days | |
| 200 μL | $527 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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| Description | Anti-HSD3B1+ HSD3B2 Polyclonal Antibody is a Rabbit antibody targeting HSD3B1+ HSD3B2. Anti-HSD3B1+ HSD3B2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat (predicted:Dog,Horse) |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (mouse adrenal gland); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (HSD3B1+HSD3B2) Polyclonal Antibody, Unconjugated (TMAB-07323) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. 2. Sample: Lane 1: Mouse Adrenal gland tissue lysates Lane 2: Rat Adrenal gland tissue lysates Primary: Anti-HSD3B1+HSD3B2 (TMAB-07323) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 41 kDa Observed band size: 41 kDa 3. Paraformaldehyde-fixed, paraffin embedded (rat adrenal gland); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (HSD3B1+HSD3B2) Polyclonal Antibody, Unconjugated (TMAB-07323) at 1:300 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded (human adrenal gland); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (HSD3B1+HSD3B2) Polyclonal Antibody, Unconjugated (TMAB-07323) at 1:300 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. 5. Paraformaldehyde-fixed, paraffin embedded (human placenta); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (HSD3B1+HSD3B2) Polyclonal Antibody, Unconjugated (TMAB-07323) at 1:300 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Endoplasmic reticulum membrane; Single-pass membrane protein. Mitochondrion membrane; Single-pass membrane protein. |
| Tissue Specificity | Placenta and skin. Predominantly expressed in mammary gland tissue. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | The protein encoded by this gene is a bifunctional enzyme that catalyzes the oxidative conversion of delta(5)-ene-3-beta-hydroxy steroid, and the oxidative conversion of ketosteroids. It plays a crucial role in the biosynthesis of all classes of hormonal steroids. This gene is predominantly expressed in the adrenals and the gonads. Mutations in this gene are associated with 3-beta-hydroxysteroid dehydrogenase, type II, deficiency. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Oct 2009] |
| Immunogen | KLH conjugated synthetic peptide: human HSD3B1+ HSD3B2 |
| Antigen Species | Human |
| Gene Name | HSD3B1 |
| Gene ID | |
| Protein Name | 3 beta-hydroxysteroid dehydrogenase/delta 5-->4-isomerase type 1 |
| Uniprot ID |
| Molecular Weight | Theoretical: 41 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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