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Anti-HPV16 E7 Polyclonal Antibody 2

(Synonyms: VE7_HPV16, Protein E7, Human papillomavirus type 16 E7, Human Papillomavirus 16(E7), Human Papilloma Virus, HPV16-E7, HPV16 E7 protein, HPV16 E7, HPV 16 E7 protein, E7 protein(HPV16), E7) Copy Product Info
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Synonyms: VE7_HPV16, Protein E7, Human papillomavirus type 16 E7, Human Papillomavirus 16(E7), Human Papilloma Virus, HPV16-E7, HPV16 E7 protein, HPV16 E7, HPV 16 E7 protein, E7 protein(HPV16), E7

Catalog No. TMAB-00886 Copy Product Info
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Anti-HPV16 E7 Polyclonal Antibody 2 is a Rabbit antibody targeting HPV16 E7. Anti-HPV16 E7 Polyclonal Antibody 2 can be used in FCM, WB.

Anti-HPV16 E7 Polyclonal Antibody 2

Pack SizePriceUSA StockGlobal StockQuantity
50 µL$2227-10 days7-10 days
100 µL$3727-10 days7-10 days
200 µL$5277-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-HPV16 E7 Polyclonal Antibody 2 is a Rabbit antibody targeting HPV16 E7. Anti-HPV16 E7 Polyclonal Antibody 2 can be used in FCM, WB.
SynonymsVE7_HPV16, Protein E7, Human papillomavirus type 16 E7, Human Papillomavirus 16(E7), Human Papilloma Virus, HPV16-E7, HPV16 E7 protein, HPV16 E7, HPV 16 E7 protein, E7 protein(HPV16), E7
Ig Type
IgG
Reactivity
HPV16
Verified Activity
1. Sample:
hela (Human) Cell Lysate at 30 μg
mcf-7 (Human) Cell Lysate at 30 μg
mda-mb-231 (Human) Cell Lysate at 30 μg
siha (Human) Cell Lysate at 30 μg
recombinant HPV16-E7 protein 100 ng
recombinant HPV16-E7 protein 50 ng
Primary: Anti-HPV16 E7 (TMAB-00886) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 11 kD
Observed band size: 17-20 kD
HPV16-E7 band size: 45 kDa
2. Sample:
Lane 1: HPV16 E7 fusion protein 0.1 μg Lysates
Lane 2: HPV16 E7 fusion protein 0.05 μg Lysates
Primary: Anti-HPV16 E7 (TMAB-00886) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 11 kDa
Observed band size: 50 kDa
3. Blank control (black line): Hela.
Primary Antibody (green line): Rabbit Anti-HPV16 E7 antibody (TMAB-00886)
Dilution: 1 μg/Test;
Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF488
Dilution: 0.5 μg/Test.
Isotype control (orange line): Normal Rabbit IgG
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at-20°C, The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
Anti-HPV16 E7 Polyclonal Antibody 2,Anti-HPV16 E7 Polyclonal Antibody 2,Anti-HPV16 E7 Polyclonal Antibody 2
Application
Recommended Dose
WB: 1:500-2000; FCM: 1ug/Test
Antibody Type
Polyclonal
Host SpeciesRabbit
ConstructionPolyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1 mg/mL
Antigen Details
Immunogen
KLH conjugated synthetic peptide: HPV16 E7 protein
Gene Name
L2
Protein Name
Minor capsid protein L2
Chemical Properties
Molecular WeightTheoretical: 11 kDa. Actual: 50 kDa.
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
Related Tags: Anti-HPV16 E7 Polyclonal Antibody 2 molecular weight