Antibody Type: Rabbit Polyclonal

Application: WB,FCM

Reactivity: Human (predicted:HPV16)

IgG

Human (predicted:HPV16)

1. Sample:
hela (Human) Cell Lysate at 30 μg
mcf-7 (Human) Cell Lysate at 30 μg
mda-mb-231 (Human) Cell Lysate at 30 μg
siha (Human) Cell Lysate at 30 μg
recombinant HPV16-E7 protein 100 ng
recombinant HPV16-E7 protein 50 ng
Primary: Anti-HPV16 E7 (TMAB-00886) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 11 kD
Observed band size: 17-20 kD
HPV16-E7 band size: 45 kDa
2. Sample:
Lane 1: HPV16 E7 fusion protein 0.1 μg Lysates
Lane 2: HPV16 E7 fusion protein 0.05 μg Lysates
Primary: Anti-HPV16 E7 (TMAB-00886) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 11 kDa
Observed band size: 50 kDa
3. Blank control (black line): Hela.
Primary Antibody (green line): Rabbit Anti-HPV16 E7 antibody (TMAB-00886)
Dilution: 1 μg/Test;
Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF488
Dilution: 0.5 μg/Test.
Isotype control (orange line): Normal Rabbit IgG
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at-20°C, The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.

WB,FCM

WB: 1:500-2000; FCM: 1ug/Test

Polyclonal

KLH conjugated synthetic peptide: HPV16 E7 protein