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Anti-HPV16 E7 Polyclonal Antibody

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Catalog No. TMAB-00885

Anti-HPV16 E7 Polyclonal Antibody is a Rabbit antibody targeting HPV16 E7. Anti-HPV16 E7 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.

Anti-HPV16 E7 Polyclonal Antibody

Anti-HPV16 E7 Polyclonal Antibody

😃Good
Catalog No. TMAB-00885
Anti-HPV16 E7 Polyclonal Antibody is a Rabbit antibody targeting HPV16 E7. Anti-HPV16 E7 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.
Pack SizePriceAvailabilityQuantity
50 μL$222 7-10 days
100 μL$374 7-10 days
200 μL$528 7-10 days
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Product Introduction

Bioactivity
Description
Anti-HPV16 E7 Polyclonal Antibody is a Rabbit antibody targeting HPV16 E7. Anti-HPV16 E7 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.
Ig Type
IgG
Reactivity
Human,HPV16
Verified Activity
1. Tissue/cell: human breast cancer; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-HPV16-E7 Polyclonal Antibody, Unconjugated (TMAB-00885) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. Blank control: Hela.
Primary Antibody (green line): Rabbit Anti-HPV16 E7 antibody (TMAB-00885)
Dilution: 1 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-PE
Dilution: 1 μg/test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at-20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
3. Sample:
Lane 1: Recombinant HPV16 E7 protein Bacterial lysate, DsbC & His
Primary: Anti-HPV16 E7 (TMAB-00885) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 11 kDa
Observed band size: 61 kDa
Application
WB,IHC-P,IHC-Fr,IF,FCM
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1ug/Test
Antibody Type
Polyclonal
Host SpeciesRabbit
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: HPV16 E7 protein
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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