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Anti-HPV16 E6 / HPV18 E6 Polyclonal Antibody is a Rabbit antibody targeting HPV16 E6 / HPV18 E6. Anti-HPV16 E6 / HPV18 E6 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $222 | 7-10 days | |
100 μL | $373 | 7-10 days | |
200 μL | $527 | 7-10 days |
Description | Anti-HPV16 E6 / HPV18 E6 Polyclonal Antibody is a Rabbit antibody targeting HPV16 E6 / HPV18 E6. Anti-HPV16 E6 / HPV18 E6 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM. |
Alias | Protein E6, Human papillomavirus type 18 E6, Human papillomavirus type 16 E6, Human Papilloma virus, HPV16+HPV18 E6, HPV16/18 protein, E6 |
Ig Type | IgG |
Reactivity | Human,HPV16/HPV18,Human Papillomavirus (HPV) |
Verified Activity | 1. Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-HPV16 E6+HPV18 E6 Polyclonal Antibody, Unconjugated (TMAB-00884) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 2. Sample: Lane 1: Recombinant HPV16 E6 protein, DsbC & His 0.1 μg Lane 2: Recombinant HPV16 E6 protein, DsbC & His 0.05 μg Primary: Anti-HPV16 E6 + HPV18 E6 (TMAB-00884) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 11 kDa Observed band size: 50 kDa 3. Blank control (black line): Hela. Primary Antibody (green line): Rabbit Anti-HPV16 E6 + HPV18 E6 antibody (TMAB-00884) Dilution: 1 μg/Test; Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF488 Dilution: 0.5 μg/Test. Isotype control (orange line): Normal Rabbit IgG Protocol The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at-20°C, The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. |
Application | WB,IHC-P,IHC-Fr,IF,FCM |
Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1ug/Test |
Antibody Type | Polyclonal |
Host Species | Rabbit |
Construction | Hybridoma Polyclonal Antibody |
Purification | Protein A purified |
Appearance | Liquid |
Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
Immunogen | KLH conjugated synthetic peptide: human HPV16 E6+ HPV18 E6 |
Antigen Species | Human |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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