Anti-HPV16 E6 / HPV18 E6 Polyclonal Antibody

Anti-HPV16 E6 / HPV18 E6 Polyclonal Antibody is a Rabbit antibody targeting HPV16 E6 / HPV18 E6. Anti-HPV16 E6 / HPV18 E6 Polyclonal Antibody can be used in FCM, IF, IHC-Fr, IHC-P, WB.

IgG

HPV16,HPV18

1. Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-HPV16 E6+HPV18 E6 Polyclonal Antibody, Unconjugated (TMAB-00884) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. Sample:
Lane 1: Recombinant HPV16 E6 protein, DsbC & His 0.1 μg
Lane 2: Recombinant HPV16 E6 protein, DsbC & His 0.05 μg
Primary: Anti-HPV16 E6 + HPV18 E6 (TMAB-00884) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 11 kDa
Observed band size: 50 kDa
3. Blank control (black line): Hela.
Primary Antibody (green line): Rabbit Anti-HPV16 E6 + HPV18 E6 antibody (TMAB-00884)
Dilution: 1 μg/Test;
Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF488
Dilution: 0.5 μg/Test.
Isotype control (orange line): Normal Rabbit IgG
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at-20°C, The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1ug/Test

Polyclonal

KLH conjugated synthetic peptide: HPV16 E6+ HPV18 E6

HPV16/HPV18

E6

Protein E6