Anti-HOXC9 Polyclonal Antibody is a Rabbit antibody targeting HOXC9. Anti-HOXC9 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human,Mouse,Rat (predicted:Cow,Sheep)

1. Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-HOXC9 Polyclonal Antibody, Unconjugated (TMAB-07255) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
2. Sample:
Lane 1: Muscle (Mouse) Tissue Lysate at 40 μg
Lane 2: Muscle (Rat) Tissue Lysate at 40 μg
Lane 3: Ear (Rat) Tissue Lysate at 40 μg
Primary: Anti-HOXC9 (TMAB-07255) at 1/200 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 29 kD
Observed band size: 40 kD

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human HOXC9

Human

HOXC9

Homeobox protein Hox-C9

Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.