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Anti-HOOK1 Polyclonal Antibody
😃Good
Catalog No. TMAB-07235
Anti-HOOK1 Polyclonal Antibody is a Rabbit antibody targeting HOOK1. Anti-HOOK1 Polyclonal Antibody can be used in WB.
Anti-HOOK1 Polyclonal Antibody
Anti-HOOK1 Polyclonal Antibody
😃Good
Catalog No. TMAB-07235
Anti-HOOK1 Polyclonal Antibody is a Rabbit antibody targeting HOOK1. Anti-HOOK1 Polyclonal Antibody can be used in WB.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $223 | 7-10 days | 7-10 days | |
| 100 μL | $372 | 7-10 days | 7-10 days | |
| 200 μL | $527 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-HOOK1 Polyclonal Antibody is a Rabbit antibody targeting HOOK1. Anti-HOOK1 Polyclonal Antibody can be used in WB. |
| Ig Type | IgG |
| Reactivity | Human (predicted:Mouse,Rat,Chicken,Dog,Pig,Cow,Horse,Rabbit,Sheep) |
| Application | |
| Recommended Dose | WB: 1:500-2000 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cytoplasm, cytoskeleton. Cytoplasm. Note=Localizes to the spermatid manchette during spermiogenesis but is not present in mature spermatozoa. Localizes to punctate cytoplasmic foci which do not appear to overlap with early or late endosomes, the endoplasmic reticulum, the Golgi complex, multivesicular bodies (MVBs), lysosomes, or mitochondria. Often found in close association with microtubules. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | Microtubules mediate the spatial organization of diverse membrane-trafficking systems. The HOOK proteins, HOOK1, HOOK2 and HOOK3, comprise a family of cytosolic coiled-coil proteins that contain conserved N-terminal domains, which attach to microtubules; and more divergent C-terminal domains, which mediate binding to organelles. HOOK1, a cytoskeletal linker protein, may play a role in endocytic membrane trafficking. It exists as a homodimer, most likely mediated through its central coiled-coil domain. HOOK1 interacts with VPS18 and is required for spermatid differentiation, in which it is most likely involved in the positioning of the manchette microtubules and the flagellum. HOOK1 localizes primarily to the cytoplasm and does not associate with the Golgi complex, unlike HOOK3, which participates in the organization of the cis-Golgi compartment. |
| Immunogen | KLH conjugated synthetic peptide: Human HOOK1 |
| Antigen Species | Human |
| Gene Name | HOOK1 |
| Gene ID | |
| Protein Name | Protein Hook homolog 1 |
| Uniprot ID | |
| Function | Required for spermatid differentiation. Probably involved in the positioning of the microtubules of the manchette and the flagellum in relation to the membrane skeleton. Component of the FTS/Hook/FHIP complex (FHF complex). The FHF complex may function to promote vesicle trafficking and/or fusion via the homotypic vesicular protein sorting complex (the HOPS complex). |
| Molecular Weight | Theoretical: 85 kDa. |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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