Anti-Homer Antibody (9P436) is a Rabbit antibody targeting Homer. Anti-Homer Antibody (9P436) can be used in WB,IHC-P,IHC-Fr,IP,IF.

IgG

9P436

Human,Mouse,Rat

1. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Homer Monoclonal Antibody, Unconjugated (TMAB-07232) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Homer Monoclonal Antibody, Unconjugated (TMAB-07232) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Homer Monoclonal Antibody, Unconjugated (TMAB-07232) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
4. (Negative control) Paraformaldehyde-fixed, paraffin embedded Mouse Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Homer Monoclonal Antibody, Unconjugated (TMAB-07232) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
5. (Negative control) Paraformaldehyde-fixed, paraffin embedded Rat Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Homer Monoclonal Antibody, Unconjugated (TMAB-07232) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
6. 25 μg total protein per lane of various lysates (see on figure) probed with Homer monoclonal antibody, unconjugated (TMAB-07232) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
7. Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Homer Monoclonal Antibody, Unconjugated (TMAB-07232) at 1: 200 overnight at 4°C. Followed by conjugated Goat Anti-Ratbbit IgG antibody (Red), DAPI (blue) was used to stain the cell nuclei.
8. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Homer Monoclonal Antibody, Unconjugated (TMAB-07232) at 1: 200 overnight at 4°C. Followed by conjugated Goat Anti-Ratbbit IgG antibody (Red), DAPI (blue) was used to stain the cell nuclei.
9. Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Homer Monoclonal Antibody, Unconjugated (TMAB-07232) at 1: 200 overnight at 4°C. Followed by conjugated Goat Anti-Ratbbit IgG antibody (Red), DAPI (blue) was used to stain the cell nuclei.

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IP: 1:20-50; IF: 1:100-500

Monoclonal

A synthesized peptide: human Homer 1

Human

HOMER1

Homer protein homolog 1

Homer is a neuronal immediate early gene that is enriched at excitatory synapses and selectively binds metabotropic glutamate receptors.