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Anti-HNRNPU Antibody (9L701) is a Rabbit antibody targeting HNRNPU. Anti-HNRNPU Antibody (9L701) can be used in WB,ICC,IF,IHC.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $298 | 7-10 days | |
100 μL | $496 | 7-10 days |
Description | Anti-HNRNPU Antibody (9L701) is a Rabbit antibody targeting HNRNPU. Anti-HNRNPU Antibody (9L701) can be used in WB,ICC,IF,IHC. |
Synonyms | U21.1, Scaffold attachment factor A, SAF-A, SAFA, SAF A, pp120, p120 nuclear protein, p120, HNRPU_HUMAN, HNRPU, hnRNPU protein, HNRNPU, hnRNP U protein, hnRNP U, Heterogeneous nuclear ribonucleoprotein U |
Ig Type | IgG |
Clone | 9L701 |
Reactivity | Human,Mouse,Rat |
Verified Activity | 1. Western blot analysis of HnRNP U on different cell lysates using anti-HnRNP U at 1/500 dilution. Positive control: Lane 1: NIH-3T3, Lane 2: SH-SY-5Y, Lane 3: K562. 2. Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-HnRNP U antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-HnRNP U antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-HnRNP U antibody. Counter stained with hematoxylin. 5. Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-HnRNP U antibody. Counter stained with hematoxylin. 6. Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-HnRNP U antibody. Counter stained with hematoxylin. 7. ICC staining HnRNP U in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 8. ICC staining HnRNP U in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 9. ICC staining HnRNP U in SHSY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
Application | |
Recommended Dose | WB: 1:500-1000; IHC: 1:50-200; ICC: 1:100-500 |
Antibody Type | Monoclonal |
Host Species | Rabbit |
Construction | Recombinant Antibody |
Purification | ProA affinity purified |
Appearance | Liquid |
Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
Research Background | DNA- and RNA-binding protein involved in several cellular processes such as nuclear chromatin organization, telomere-length regulation, transcription, mRNA alternative splicing and stability, Xist-mediated transcriptional silencing and mitotic cell progression. Plays a role in the regulation of interphase large-scale gene-rich chromatin organization through chromatin-associated RNAs (caRNAs) in a transcription-dependent manner, and thereby maintains genomic stability. Plays a role as a RNA polymerase II (Pol II) holoenzyme transcription regulator. Promotes transcription initiation by direct association with the core-TFIIH basal transcription factor complex for the assembly of a functional pre-initiation complex with Pol II in a actin-dependent manner. Blocks Pol II transcription elongation activity by inhibiting the C-terminal domain (CTD) phosphorylation of Pol II and dissociates from Pol II pre-initiation complex prior to productive transcription elongation. Positively regulates CBX5-induced transcriptional gene silencing and retention of CBX5 in the nucleus. |
Conjucates | Unconjugated |
Immunogen | Recombinant Protein |
Uniprot ID |
Molecular Weight | Theoretical: 120 kDa. |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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