Anti-HNRNPF Antibody (1W7) is a Mouse antibody targeting HNRNPF. Anti-HNRNPF Antibody (1W7) can be used in WB,ICC/IF.

IgG1, Kappa

1W7

Human (predicted:Mouse,Rat)

1. Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:5000
Primary ab incubation condition: room temperature 2 h
Secondary ab: Goat Anti-Mouse IgG H&L (HRP)
Lysate: 293, A20, BRL, Daudi
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: 46 kDa
Observed MW: 46 kDa
2. Cell line: HepG2
Fixative: 4% Paraformaldehyde
Permeabilization: 0.1% TritonX-100
Primary ab dilution: 1:50
Primary incubation condition: 4°C overnight
Secondary ab: Goat Anti-Mouse IgG
Nuclear counter stain: DAPI (Blue)
Counter stain: Tubulin (Red)
Comment: Color green is the positive signal for PTM-6026

WB: 1:1000-5000; ICC/IF: 1:100-500

Monoclonal

HNRNPF

Heterogeneous nuclear ribonucleoprotein F

Component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. Plays a role in the regulation of alternative splicing events. Binds G-rich sequences in pre-mRNAs and keeps target RNA in an unfolded state.