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Anti-hnRNP F Polyclonal Antibody

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Catalog No. TMAB-07215

Anti-hnRNP F Polyclonal Antibody is a Rabbit antibody targeting hnRNP F. Anti-hnRNP F Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.

Anti-hnRNP F Polyclonal Antibody

Anti-hnRNP F Polyclonal Antibody

Copy Product Info
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Catalog No. TMAB-07215
Anti-hnRNP F Polyclonal Antibody is a Rabbit antibody targeting hnRNP F. Anti-hnRNP F Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
Pack SizePriceUSA WarehouseGlobal WarehouseQuantity
50 μL$2237-10 days7-10 days
100 μL$3747-10 days7-10 days
200 μL$5277-10 days7-10 days
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction

Bioactivity
Description
Anti-hnRNP F Polyclonal Antibody is a Rabbit antibody targeting hnRNP F. Anti-hnRNP F Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
Ig Type
IgG
Reactivity
Mouse (predicted:Human,Rat,Dog,Pig,Rabbit)
Verified Activity
1. Tissue/cell: mouse embryo tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-hnRNP F Polyclonal Antibody, Unconjugated (TMAB-07215) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
2. Tissue/cell: mouse pancreas tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-hnRNP F Polyclonal Antibody, Unconjugated (TMAB-07215) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
Application
Recommended Dose
IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationNucleus, nucleoplasm.
Tissue SpecificityExpressed ubiquitously.
ConstructionPolyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundhnRNP F is part of the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). These are RNA binding proteins that complex with heterogeneous nuclear RNA (hnRNA) and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. hnRNP F binds to RNAs which have guanosine-rich sequences.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human hnRNP F
Antigen Species
Human
Gene Name
HNRNPF
Gene ID
Protein Name
Heterogeneous nuclear ribonucleoprotein F
Uniprot ID
Function
Component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. Plays a role in the regulation of alternative splicing events. Binds G-rich sequences in pre-mRNAs and keeps target RNA in an unfolded state.
Chemical Properties
Molecular WeightTheoretical: 46 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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