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Anti-hnRNP A3 Polyclonal Antibody

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Catalog No. TMAB-07210

Anti-hnRNP A3 Polyclonal Antibody is a Rabbit antibody targeting hnRNP A3. Anti-hnRNP A3 Polyclonal Antibody can be used in IHC-P,IHC-Fr,ICC/IF,IF.

Anti-hnRNP A3 Polyclonal Antibody

Anti-hnRNP A3 Polyclonal Antibody

Copy Product Info
😃Good
Catalog No. TMAB-07210
Anti-hnRNP A3 Polyclonal Antibody is a Rabbit antibody targeting hnRNP A3. Anti-hnRNP A3 Polyclonal Antibody can be used in IHC-P,IHC-Fr,ICC/IF,IF.
Pack SizePriceUSA WarehouseGlobal WarehouseQuantity
50 μL$2217-10 days7-10 days
100 μL$3727-10 days7-10 days
200 μL$5277-10 days7-10 days
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction

Bioactivity
Description
Anti-hnRNP A3 Polyclonal Antibody is a Rabbit antibody targeting hnRNP A3. Anti-hnRNP A3 Polyclonal Antibody can be used in IHC-P,IHC-Fr,ICC/IF,IF.
Ig Type
IgG
Reactivity
Human,Mouse (predicted:Rat,Dog,Cow,Horse,Rabbit)
Verified Activity
1. Tissue/cell: HepG2 cells; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-hmRNPA3 Polyclonal Antibody, Unconjugated (TMAB-07210) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
2. Tissue/cell: mouse intestine tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-hnRNP A3 Polyclonal Antibody, Unconjugated (TMAB-07210) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
Application
Recommended Dose
IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationNuclear
ConstructionPolyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundHeterogeneous nuclear ribonucleoproteins (hnRNPs) constitute a set of poly-peptides that contribute to mRNA transcription and pre-mRNA processing as well as mature mRNA transport to the cytoplasm and translation. They also bind heterogeneous nuclear RNA (hnRNA), which are the transcripts produced by RNA polymerase II. There are approximately 20 known hnRNP proteins, and their complexes are the major constituents of the spliceosome. The majority of hnRNP protein components are localized to the nucleus; however some shuttle between the nucleus and the cytoplasm. The A/B subfamily of hnRNPs include A1, A2/B1, A3 and A0, and in Xenopus, hnRNP A1, A2 and A3 are ubiquitously expressed throughout development as well as in adult tissues. hnRNP A1 and A2/B1 regulate the processing of pre-mRNA by directly antagonizing the association of various splicing factors and by influencing the splice site selection on pre-mRNA. The hnRNP A0 gene is distinct from the other A/B family members, and it encodes a low-abundance protein, which is implicated in mRNA stability.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human hnRNP A3
Antigen Species
Human
Gene Name
HNRNPA3
Gene ID
Protein Name
Heterogeneous nuclear ribonucleoprotein A3
Uniprot ID
Function
HNRPA3 plays a role in cytoplasmic trafficking of RNA. It binds to the cis-acting response element(A2RE) and may be involved in pre-mRNA splicing.
Chemical Properties
Molecular WeightTheoretical: 40 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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