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Anti-HNF4A Antibody (3W706)

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Catalog No. TMAB-07205

Anti-HNF4A Antibody (3W706) is a Rabbit antibody targeting HNF4A. Anti-HNF4A Antibody (3W706) can be used in WB,ICC/IF.

Anti-HNF4A Antibody (3W706)

Anti-HNF4A Antibody (3W706)

Copy Product Info
😃Good
Catalog No. TMAB-07205
Anti-HNF4A Antibody (3W706) is a Rabbit antibody targeting HNF4A. Anti-HNF4A Antibody (3W706) can be used in WB,ICC/IF.
Pack SizePriceUSA WarehouseGlobal WarehouseQuantity
25 μL$1517-10 days7-10 days
50 μL$2637-10 days7-10 days
100 μL$4737-10 days7-10 days
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction

Bioactivity
Description
Anti-HNF4A Antibody (3W706) is a Rabbit antibody targeting HNF4A. Anti-HNF4A Antibody (3W706) can be used in WB,ICC/IF.
Ig Type
IgG
Clone
3W706
Reactivity
Human
Verified Activity
1. 4% Paraformaldehyde-fixed HepG2 (H) cell; Triton X-100 at r. T. for 20 min; Antibody incubation with (HNF4A) monoclonal Antibody, unconjugated (TMAB-07205) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green) at 37°C for 90 min, DAPI (blue) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
2. 25 μg total protein per lane of various lysates (see on figure) probed with HNF4A monoclonal antibody, unconjugated (TMAB-07205) at 1: 2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
Application
Recommended Dose
WB: 1:500-2000; ICC/IF: 1:50-200
Antibody Type
Monoclonal
Host SpeciesRabbit
Subcellular LocalizationNucleus.
ConstructionRecombinant Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation10mM phosphate buffered saline (pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.
Research BackgroundTranscriptionally controlled transcription factor. Binds to DNA sites required for the transcription of alpha 1-antitrypsin, apolipoprotein CIII, transthyretin genes and HNF1-alpha. May be essential for development of the liver, kidney and intestine.
Antigen Details
Immunogen
A synthesized peptide: human HNF 4 alpha
Antigen Species
Human
Gene Name
HNF4A
Gene ID
Protein Name
Hepatocyte nuclear factor 4-alpha
Uniprot ID
Function
Transcriptionally controlled transcription factor. Binds to DNA sites required for the transcription of alpha 1-antitrypsin, apolipoprotein CIII, transthyretin genes and HNF1-alpha. May be essential for development of the liver, kidney and intestine.
Chemical Properties
Molecular WeightTheoretical: 53 kDa. Actual: 53 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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