Anti-HMX2 Polyclonal Antibody is a Rabbit antibody targeting HMX2. Anti-HMX2 Polyclonal Antibody can be used in FCM.

IgG

Human (predicted:Mouse,Chicken,Dog,Pig,Cow,Horse,Rabbit)

Blank control: Hela.
Primary Antibody (green line): Rabbit Anti-HMX2 antibody (TMAB-07198)
Dilution: 1 μg/Test;
Secondary Antibody: Goat anti-rabbit IgG-FITC
Dilution: 0.5 μg/Test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20min at -20℃. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

FCM: 1μg/Test

Polyclonal

KLH conjugated synthetic peptide: human HMX2

Human

HMX2

Homeobox protein HMX2

Transcription factor involved in specification of neuronal cell types and which is required for inner ear and hypothalamus development.