Antibody Type: Rabbit Polyclonal

Application: WB,IHC-P,IHC-Fr,IF

Reactivity: Human,Mouse,Rat,Pig (predicted:Chicken,Dog,Cow,Horse,Rabbit,Sheep,GuineaPig)

IgG

Human,Mouse,Rat,Pig (predicted:Chicken,Dog,Cow,Horse,Rabbit,Sheep,GuineaPig)

1. Tissue/cell: rat lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-Heme Oxygenase 1 Polyclonal Antibody, Unconjugated (TMAB-00875) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. Sample:
Lane 1: HepG2 (Human) Cell Lysate at 30 μg
Lane 2: A549 (Human) Cell Lysate at 30 μg
Lane 3: DU145 (Human) Cell Lysate at 30 μg
Lane 4: H9C2 (Rat) Cell Lysate at 30 μg
Lane 5: 293T (Human) Cell Lysate at 30 μg
Primary: Anti-Heme Oxygenase 1 (TMAB-00875) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 32 kDa
Observed band size: 35 kDa

WB,IHC-P,IHC-Fr,IF

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human HO-1

Human

3162

Response to hypoxia,Inflammatory mediators,Platelets,Endothelium,NFkB pathway,Hypoxia,Cancer,Heart disease,Neurodegenerative disease,Alzheimer's disease,NFkB Pathway