Anti-HMOX1 Polyclonal Antibody is a Rabbit antibody targeting HMOX1. Anti-HMOX1 Polyclonal Antibody can be used in IF, IHC-Fr, IHC-P, WB.

IgG

Human,Rat (predicted:Mouse,Chicken,Dog,Pig,Cow,Horse,Rabbit,Sheep,GuineaPig)

1. Tissue/cell: rat lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-Heme Oxygenase 1 Polyclonal Antibody, Unconjugated (TMAB-00875) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. Sample:
Lane 1: HepG2 (Human) Cell Lysate at 30 μg
Lane 2: A549 (Human) Cell Lysate at 30 μg
Lane 3: DU145 (Human) Cell Lysate at 30 μg
Lane 4: H9C2 (Rat) Cell Lysate at 30 μg
Lane 5: 293T (Human) Cell Lysate at 30 μg
Primary: Anti-Heme Oxygenase 1 (TMAB-00875) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 32 kDa
Observed band size: 35 kDa

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human HO-1

Human

HMOX1

Heme oxygenase 1

Response to hypoxia,Inflammatory mediators,Platelets,Endothelium,NFkB pathway,Hypoxia,Cancer,Heart disease,Neurodegenerative disease,Alzheimer's disease,NFkB Pathway

Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed.