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Anti-HMGB4 Polyclonal Antibody
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Catalog No. TMAB-07185
Anti-HMGB4 Polyclonal Antibody is a Rabbit antibody targeting HMGB4. Anti-HMGB4 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-HMGB4 Polyclonal Antibody
Anti-HMGB4 Polyclonal Antibody
Copy Product Info😃Good
Catalog No. TMAB-07185
Anti-HMGB4 Polyclonal Antibody is a Rabbit antibody targeting HMGB4. Anti-HMGB4 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $223 | 7-10 days | 7-10 days | |
| 100 μL | $373 | 7-10 days | 7-10 days | |
| 200 μL | $527 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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| Description | Anti-HMGB4 Polyclonal Antibody is a Rabbit antibody targeting HMGB4. Anti-HMGB4 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Reactivity | Mouse,Rat (predicted:Human,Dog,Pig,Cow) |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (rat testis tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (HMGB4) Polyclonal Antibody, Unconjugated (TMAB-07185) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. 2. Sample: Testis (Rat) Lysate at 40 μg Primary: Anti-HMGB4 (TMAB-07185) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 22 kD Observed band size: 22 kD 3. Paraformaldehyde-fixed, paraffin embedded (mouse testis tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (HMGB4) Polyclonal Antibody, Unconjugated (TMAB-07185) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Nucleus (By similarity). Chromosome (By similarity). |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | High Mobility Group (HMG) proteins, a group of chromosomal proteins common to all eukaryotes, bind DNA in a non-sequence-specific fashion to promote chromatin function and gene regulation, helping with transcription, replication, recombination, and DNA repair. HMGB4 is HMG2-like, isoform 2, high-mobility group box 4. This gene can be found on Chromosome 1. HMGB4 contains two HMG-box regions, and is found in a variety of eukaryotic chromosomal proteins. HMGB proteins are phosphorylated to various extents. The existence of differentially modified forms increases the number of distinct HMGB protein variants in plant chromatin that may be adapted to certain functions. |
| Immunogen | KLH conjugated synthetic peptide: human HMGB4 |
| Antigen Species | Human |
| Gene Name | HMGB4 |
| Gene ID | |
| Protein Name | High mobility group protein B4 |
| Uniprot ID |
| Molecular Weight | Theoretical: 22 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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