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Anti-HMGA1 Polyclonal Antibody
😃Good
Catalog No. TMAB-00869
Anti-HMGA1 Polyclonal Antibody is a Rabbit antibody targeting HMGA1. Anti-HMGA1 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
Anti-HMGA1 Polyclonal Antibody
Anti-HMGA1 Polyclonal Antibody
😃Good
Catalog No. TMAB-00869
Anti-HMGA1 Polyclonal Antibody is a Rabbit antibody targeting HMGA1. Anti-HMGA1 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $221 | 7-10 days | |
| 100 μL | $373 | 7-10 days | |
| 200 μL | $528 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Antibody Type: Rabbit Polyclonal Application: IHC-P,IHC-Fr,IF Reactivity: Human (predicted:Mouse,Rat,Chicken,Cow) |
| Ig Type | IgG |
| Reactivity | Human (predicted:Mouse,Rat,Chicken,Cow) |
| Verified Activity | 1. Tissue/cell: human glioma tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-HMGA1/HMG-I/HMG-Y Polyclonal Antibody, Unconjugated (TMAB-00869) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 2. Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-HMGA1/HMG-I/HMG-Y Polyclonal Antibody, Unconjugated (TMAB-00869) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. |
| Application | IHC-P,IHC-Fr,IF |
| Recommended Dose | IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Nucleus. Chromosome. |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | HMGA1 (HMGI/Y; high mobility group AT-hook 1) is a non-histone protein involved in many cellular processes, including regulation of inducible gene transcription, integration of retroviruses into chromosomes, and the metastatic progression of cancer cells. There are two splice isoforms of HMGA1 - the full length HMGA1a/HMGI and the shortened form HMGA1b/HMGY. The encoded protein preferentially binds to the minor groove of A+T-rich regions in double-stranded DNA. It has little secondary structure in solution but assumes distinct conformations when bound to substrates such as DNA or other proteins. HMGA1 is frequently acetylated and is found in the nucleus. Interacts with HIV-1 pre-integration complex. |
| Immunogen | KLH conjugated synthetic peptide: human HMGA1 |
| Antigen Species | Human |
| Gene ID | 3159 |
| Uniprot ID | |
| Biology Area | Tumor biomarkers,DNA / RNA binding,Base Excision Repair,Transcription Factors,Host Virus Interaction |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
Sci Citations
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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