Anti-HLTF Antibody (1B226) is a Rabbit antibody targeting . Anti-HLTF Antibody (1B226) can be used in WB,FCM.

IgG

1B226

Human

1. 25 ug total protein per lane of various lysates (see on figure) probed with HLTF monoclonal antibody, unconjugated (TMAB-07173) at 1: 1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
2. The Hela (H) cells were fixed with 4% PFA (10 min at r. T.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-HLTF antibody (TMAB-07173, 1: 100); Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed.

WB=1:500-2000,FCM=1:50-100

Monoclonal

A synthesized peptide: human HLTF

Human

HLTF

Helicase-like transcription factor

Has both helicase and E3 ubiquitin ligase activities. Possesses intrinsic ATP-dependent nucleosome-remodeling activity; This activity may be required for transcriptional activation or repression of specific target promoters (By similarity). These may include the SERPINE1 and HIV-1 promoters and the SV40 enhancer, to which this protein can bind directly. Plays a role in error-free postreplication repair (PRR) of damaged DNA and maintains genomic stability through acting as a ubiquitin ligase for 'Lys-63'-linked polyubiquitination of chromatin-bound PCNA.