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Anti-HLA G Polyclonal Antibody

Catalog No. TMAB-07155 Copy Product Info
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Anti-HLA G Polyclonal Antibody is a Rabbit antibody targeting HLA G. Anti-HLA G Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-HLA G Polyclonal Antibody

Copy Product Info
🥰Excellent
Catalog No. TMAB-07155

Anti-HLA G Polyclonal Antibody is a Rabbit antibody targeting HLA G. Anti-HLA G Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-HLA G Polyclonal Antibody
Pack SizePriceUSA StockGlobal StockQuantity
50 μL$2237-10 days7-10 days
100 μL$3737-10 days7-10 days
200 μL$5287-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-HLA G Polyclonal Antibody is a Rabbit antibody targeting HLA G. Anti-HLA G Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG
Reactivity
Human,Rat
Verified Activity
1. Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-HLA B/HLA G Polyclonal Antibody, Unconjugated (TMAB-07155) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
2. Sample:
Brain (Rat) Lysate at 30 μg
Kidney (Rat) lysate at 30 μg
Primary: Anti-HLA G (TMAB-07155) at 1/200 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG at 1/3000 dilution
Predicted band size: 35 kD
Observed band size: 35 kD
Application
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationMembrane; Single-pass type I membrane protein.
Tissue SpecificityExpressed in trophoblasts.
ConstructionPolyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundHLA-G belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-G is expressed on fetal derived placental cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domain, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exon 6 encodes the cytoplasmic tail. [provided by RefSeq, Jul 2008]
Antigen Details
Immunogen
KLH conjugated synthetic peptide: the middle of human HLA-G
Antigen Species
Human
Gene Name
HLA-G
Gene ID
Protein Name
HLA class I histocompatibility antigen, alpha chain G
Uniprot ID
Chemical Properties
Molecular WeightTheoretical: 35 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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