Anti-HLA E Polyclonal Antibody 2 is a Rabbit antibody targeting HLA E. Anti-HLA E Polyclonal Antibody 2 can be used in WB,FCM.

IgG

Human

1. Blank control: A549.
Primary Antibody (green line): Rabbit Anti-HLAE antibody (TMAB-07151)
Dilution: 3 μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-PE
Dilution: 3 μg /test.
Protocol
The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
2. Sample: K562 Cell (Human) Lysate at 30 μg
Primary: Anti-HLA e at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 37 kD
Observed band size: 35 kD
3. Sample: Raji Cell (Human) Lysate at 30 μg
Primary: Anti-HLA e at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 37 kD
Observed band size: 35 kD

WB: 1:500-2000; FCM: 3μg/Test

Polyclonal

KLH conjugated synthetic peptide: human HLA E

Human

HLA-E

HLA class I histocompatibility antigen, alpha chain E

Preferably binds to a peptide derived from the signal sequence of most HLA-A, -B, -C and -G molecules.