Anti-HLA-E Antibody (8J391) is a Mouse antibody targeting HLA-E. Anti-HLA-E Antibody (8J391) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.

IgG2a, Kappa

8J391

Human

1. Cell line: THP-1
Fixative: 4% Paraformaldehyde
Permeabilization: 0.1% TritonX-100
Primary Ab dilution: 1:50
Primary incubation condition: 4°C overnight
Secondary Ab: Goat Anti-Mouse IgG
Nuclear counter stain: DAPI (Blue)
Comment: Color green is the positive signal for TMAB-07171
2. Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 2 hours at room temperature
Secondary Ab: Goat Anti-Mouse IgG H&L (HRP)
Lysate: 1: THP-1, 2: Raji
Protein loading quantity: 20 μg
Exposure time: 3 s
Predicted MW: 40 kDa
Observed MW: 40 kDa
3. Tissue: Human tonsil
Section type: Formalin fixed & Paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:100
Primary Ab incubation condition: 1 hour at room temperature
Secondary Ab: SP Kit (Mouse)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for TMAB-07171

WB: 1:500-1000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:100-500; IF: 1:100-500

Monoclonal

HLA-E

HLA class I histocompatibility antigen, alpha chain E

Preferably binds to a peptide derived from the signal sequence of most HLA-A, -B, -C and -G molecules.