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Anti-HLA-DRA Antibody (9X138)
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Catalog No. TMAC-01976
Alias MHC class II antigen DRA, HLA-DRA1, HLA-DRA, HLA class II histocompatibility antigen, DR alpha chain, DR alpha chain
Anti-HLA-DRA Antibody (9X138) is a Rabbit antibody targeting HLA-DRA. Anti-HLA-DRA Antibody (9X138) can be used in WB,ICC/IF,IHC,FCM.
Anti-HLA-DRA Antibody (9X138)
Anti-HLA-DRA Antibody (9X138)
😃Good
Catalog No. TMAC-01976Alias MHC class II antigen DRA, HLA-DRA1, HLA-DRA, HLA class II histocompatibility antigen, DR alpha chain, DR alpha chain
Anti-HLA-DRA Antibody (9X138) is a Rabbit antibody targeting HLA-DRA. Anti-HLA-DRA Antibody (9X138) can be used in WB,ICC/IF,IHC,FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $298 | 7-10 days | |
| 100 μL | $496 | 7-10 days |
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Chemical Properties
| Description | Anti-HLA-DRA Antibody (9X138) is a Rabbit antibody targeting HLA-DRA. Anti-HLA-DRA Antibody (9X138) can be used in WB,ICC/IF,IHC,FCM. |
| Synonyms | MHC class II antigen DRA, HLA-DRA1, HLA-DRA, HLA class II histocompatibility antigen, DR alpha chain, DR alpha chain |
| Ig Type | IgG |
| Reactivity | Human,Rat |
| Verified Activity | 1. Western blot analysis of HLA-DR on Daudi cells lysates using anti-HLA-DR antibody at 1/1,000 dilution. 2. Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-HLA-DR antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-HLA-DR antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-HLA-DR antibody. Counter stained with hematoxylin. 5. Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-HLA-DR antibody. Counter stained with hematoxylin. 6. Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-HLA-DR antibody. Counter stained with hematoxylin. 7. ICC staining HLA-DR in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 8. ICC staining HLA-DR in B16-F1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 9. Flow cytometric analysis of Jurkat cells with HLA-DR antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
| Application | |
| Recommended Dose | WB: 1:1000-5000; IHC: 1:50-200; ICC/IF: 1:50-200; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | Major histocompatibility complex (MHC) class II molecules destined for presentation to CD4+ helper T cells is determined by two key events. These events include the dissociation of class II-associated invariant chain peptides (CLIP) from an antigen binding groove in MHC II α/β dimers through the activity of MHC molecules HLA-DM and -DO, and subsequent peptide antigen binding. Accumulating in endosomal/lysosomal compartments and on the surface of B cells, HLA-DM, -DO molecules regulate the dissociation of CLIP and the subsequent binding of exogenous peptides to HLA class II molecules (HLA-DR, -DQ and -DP) by sustaining a conformation that favors peptide exchange. RFLP analysis of HLA-DM genes from rheumatoid arthritis (RA) patients suggests that certain polymorphisms are genetic factors for RA susceptibility. HLA-B belongs to the HLA class I heavy chain paralogs. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. HLA-B and -C can form heterodimers consisting of a membrane anchored heavy chain and a light chain (β-2-Microglobulin). Polymorphisms yield hundreds of HLA-B and -C alleles. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Uniprot ID |
| Molecular Weight | Theoretical: 29 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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