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Anti-HLA-A Antibody (2M442) (Synonyms: HLAA)
Catalog No. TMAC-02580 Copy Product Info
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Anti-HLA-A Antibody (2M442) is a Rabbit antibody targeting HLA-A. Anti-HLA-A Antibody (2M442) can be used in FCM,ICC/IF,IHC,WB.
Anti-HLA-A Antibody (2M442)
Copy Product Info🥰Excellent
Catalog No. TMAC-02580
Synonyms HLAA
Anti-HLA-A Antibody (2M442) is a Rabbit antibody targeting HLA-A. Anti-HLA-A Antibody (2M442) can be used in FCM,ICC/IF,IHC,WB.
Anti-HLA-A Antibody
(2M442)
(2M442)
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 50 μL | $296 | 7-10 days | 7-10 days | |
| 100 μL | $498 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction
Bioactivity
Related Conjugates and Formulations
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Chemical Properties
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| Description | Anti-HLA-A Antibody (2M442) is a Rabbit antibody targeting HLA-A. Anti-HLA-A Antibody (2M442) can be used in FCM,ICC/IF,IHC,WB. |
| Synonyms | HLAA |
| Ig Type | IgG |
| Clone | 2M442 |
| Reactivity | Human |
| Verified Activity | 1. Western blot analysis of MHC class I on different lysates using anti-MHC class I antibody at 1/1,000 dilution. Positive control: Lane 1: Raji, Lane 2: THP-1. 2. Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-MHC class I antibody. Counter stained with hematoxylin. 3. ICC staining MHC class I in SH-SY-5Y cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 4. Flow cytometric analysis of THP-1 cells with MHC class I antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.     |
| Application | |
| Recommended Dose | WB: 1:1000-5000; IHC: 1:50-200; ICC/IF: 1:100-500; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | Major histocompatibility complex (MHC) molecules, also designated human leukocyte antigen (HLA) molecules, are cell-surface receptors that bind foreign peptides and present them to T lymphocytes. MHC class I molecules consist of two polypeptide chains, an a or heavy chain, and β-2-Microglobulin, a non-covalently associated protein. Cytotoxic T lymphocytes bind antigenic peptides presented by MHC class I molecules. Antigens that bind to MHC class I molecules are typically 8-10 residues in length and are stabilized in a peptide binding groove. MHC class II molecules are encoded by polymorphic MHC genes and consist of a non-covalent complex of an a and b chain. Helper T lymphocytes bind antigenic peptides presented by MHC class II molecules. MHC class II molecules bind 13-18 amino acid antigenic peptides. Accumulating in endosomal/lysosomal compartments and on the surface of B cells, HLA-DM and -DO molecules regulate binding of exogenous peptides to class II molecules (HLA-DR) by sustaining a conformation that favors peptide exchange. The differential structural properties of MHC class I and class II molecules account for their respective roles in activating different populations of T lymphocytes. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Uniprot ID |
| Molecular Weight | Theoretical: 41 kDa. |
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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