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Anti-HK1 Antibody (7G677)
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Catalog No. TMAC-01816
Alias HK1, Hexokinase-A, Hexokinase-1, Hexokinase type I (HK I), Brain form hexokinase
Anti-HK1 Antibody (7G677) is a Rabbit antibody targeting HK1. Anti-HK1 Antibody (7G677) can be used in WB,ICC/IF,IHC,IP,FCM.
Anti-HK1 Antibody (7G677)
Anti-HK1 Antibody (7G677)
😃Good
Catalog No. TMAC-01816Alias HK1, Hexokinase-A, Hexokinase-1, Hexokinase type I (HK I), Brain form hexokinase
Anti-HK1 Antibody (7G677) is a Rabbit antibody targeting HK1. Anti-HK1 Antibody (7G677) can be used in WB,ICC/IF,IHC,IP,FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $298 | 7-10 days | |
| 100 μL | $496 | 7-10 days |
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Antigen Details
Chemical Properties
| Description | Anti-HK1 Antibody (7G677) is a Rabbit antibody targeting HK1. Anti-HK1 Antibody (7G677) can be used in WB,ICC/IF,IHC,IP,FCM. |
| Synonyms | HK1, Hexokinase-A, Hexokinase-1, Hexokinase type I (HK I), Brain form hexokinase |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Western blot analysis of Hexokinase 1 on different lysates using anti-Hexokinase 1 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela, Lane 2: 293, Lane 3: MCF-7, Lane 4: HepG2. 2. Immunohistochemical analysis of paraffin-embedded mouse skin tissue using anti-Hexokinase 1 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-Hexokinase 1 antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Hexokinase 1 antibody. Counter stained with hematoxylin. 5. Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Hexokinase 1 antibody. Counter stained with hematoxylin. 6. Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Hexokinase 1 antibody. Counter stained with hematoxylin. 7. Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Hexokinase 1 antibody. Counter stained with hematoxylin. 8. ICC staining Hexokinase 1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 9. ICC staining Hexokinase 1 in CRC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 10. ICC staining Hexokinase 1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 11. Flow cytometric analysis of K562 cells with Hexokinase 1 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
| Application | |
| Recommended Dose | WB: 1:1000-2000; IHC: 1:50-200; ICC/IF: 1:50-200; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | The hexokinases utilize Mg-ATP as a phosphoryl donor to catalyze the first step of intracellular glucose metabolism, the conversion of glucose to glucose-6-phosphate. Four hexokinase isoenzymes have been identified, including hexokinase I (HXK I), hexokinase II (HXK II), hexokinase III (HXK III) and hexokinase IV (HXK IV, also designated glucokinase or GCK). Hexokinases I-III each contain an N-terminal cluster of hydrophobic amino acids. Glucokinase lacks the N-terminal hydrophobic cluster. The hydrophobic cluster is thought to be necessary for membrane binding. This is substantiated by the finding that glucokinase has lower affinity for glucose than do the other hexokinases. HXK I has been shown to be expressed in brain, kidney and heart tissues as well as in hepatoma cell lines. HXK II is involved in the uptake and utilization of glucose by adipose and skeletal tissues. Of the hexokinases, HXK III has the highest affinity for glucose. Glucokinase is expressed in pancreatic beta cells where it functions as a glucose sensor, determining the ??set point?? for insulin secretion. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Uniprot ID |
| Molecular Weight | Theoretical: 102 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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