Anti-HIV TAT specific factor 1 Antibody (3D684) is a Rabbit antibody targeting HIV TAT specific factor 1. Anti-HIV TAT specific factor 1 Antibody (3D684) can be used in FCM, ICC/IF, IP, WB.

IgG

3D684

Human,Mouse

1. 25 ug total protein per lane of various lysates (see on figure) probed with HIV TAT specific factor 1 monoclonal antibody, unconjugated (TMAB-07145) at 1: 1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
2. The Hela (H) cells were fixed with 4% PFA (10 min at r. T.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-Lysine tRNA synthetase antibody (TMAB-07145, 1: 100); Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed.

FCM=1:50-100; ICC/IF=1:50-200; IP=1:20-50; WB=1:500-2000

Monoclonal

A synthesized peptide: human Tat SF1

Human

HTATSF1

17S U2 SnRNP complex component HTATSF1

HIV TAT specific factor(a.k.a. HTATSF1, Tat-SF1 or HTSF1) is an 86 kDa general transcription factor that plays a role in the process of transcription elongation. However, in HIV-infected cells, this factor is up-regulated by HIV Nef and gp120 and acts as a co-factor for the Tat-enhanced transcription of the HIV virus.