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Anti-Histone H3 Antibody (3W197)
😃Good
Catalog No. TMAY-00020
Anti-Histone H3 Antibody (3W197) is a Mouse antibody targeting Histone H3. Anti-Histone H3 Antibody (3W197) can be used in WB,IHC-P.
Anti-Histone H3 Antibody (3W197)
Anti-Histone H3 Antibody (3W197)
😃Good
Catalog No. TMAY-00020
Anti-Histone H3 Antibody (3W197) is a Mouse antibody targeting Histone H3. Anti-Histone H3 Antibody (3W197) can be used in WB,IHC-P.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 20 μL | $68 | 7-10 days | |
| 100 μL | $139 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Mouse Monoclonal Application: WB, IHC-P Reactivity: Human |
| Ig Type | Mouse IgG2b |
| Clone | 3W197 |
| Reactivity | Human |
| Verified Activity | 1. Immunochemical staining of human histone H3 in human mammary gland with mouse monoclonal antibody (formalin-fixed paraffin embedded sections). Positive staining was localized to nucleus. 2. Immunochemical staining of human histone H3 in human colon carcinoma with mouse monoclonal antibody (formalin-fixed paraffin embedded sections). Positive staining was localized to nucleus. The left panel: tissue incubated with primary antibody;The right panel: tissue incubated with the mixture of primary antibody and antigen (polypeptide). 3. Anti-Histone H3 mouse monoclonal antibody at 1:2000 dilution. -Lane A: Hela Whole Cell Lysate. -Lane B: Jurkat Whole Cell Lysate. -Lane C: 293 Whole Cell lysate. -Lysates/proteins at 30 μg per Lane. -Secondary -Goat Anti-Mouse IgG H&L (Dylight800) at 1/7500 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size: 18 kDa. -Observed band size: 18 kDa. 4. Anti-Histone H3 mouse monoclonal antibody at 1:10000, 1:20000, 1:50000 dilution -Lysates/proteins at 30 μg per Lane. -Secondary -Rabbit Anti-Mouse IgG F(ab)2/HRP at 1/10000 dilution. -Developed using the ECL technique. -Performed under reducing conditions. -Predicted band size: 18 kDa. -Observed band size:18 kDa. 5. Anti-Histone H3 mouse monoclonal antibody at 1:20000 dilution -Lysates/proteins at 30 μg per Lane. -Secondary -Rabbit Anti-Mouse IgG F(ab)2/HRP at 1/10000 dilution. -Developed using the ECL technique. -Performed under reducing conditions. -Predicted band size: 18 kDa. -Observed band size:18 kDa. 6. Anti-Histone H3 mouse monoclonal antibody at 1:20000 dilution -Lysates/proteins at 30 μg per Lane. -Secondary -Rabbit Anti-Mouse IgG F(ab)2/HRP at 1/10000 dilution. -Developed using the ECL technique. -Performed under reducing conditions. -Predicted band size: 18 kDa. -Observed band size:18 kDa. 7. Anti-Histone H3 mouse monoclonal antibody at 1:20000 dilution -Lysates/proteins at 30 μg per Lane. -Secondary -Rabbit Anti-Mouse IgG F(ab)2/HRP at 1/10000 dilution. -Developed using the ECL technique. -Performed under reducing conditions. -Predicted band size: 18 kDa. -Observed band size:18 kDa. 8. Anti-Histone H3 mouse monoclonal antibody at 1:1000 dilution. -Lane A: Histone H3 konckout Hela Whole Cell Lysate. -Lane B: Hela Whole Cell lysate. -Lysates/proteins at 10 μg per lane. -Secondary -Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution. -Developed using the ECL technique. -Performed under reducing conditions. -Predicted band size:18 kDa. -Observed band size:18 kDa(Validation Experiment) |
| Application | WB,IHC-P |
| Recommended Dose | WB: 1:1000-1:100000; IHC-P: 1/50-1/1000 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Construction | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with a synthetic peptide corresponding to the C-terminus of the human Histone H3. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | Histone H3 is a histone protein. Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. They play a central role in transcription regulation, DNA repair, DNA replication, and chromosomal stability. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. Histone H3 is characterized by a main globular domain and a long N-terminal tail. It is involved with the structure of the nucleosomes of the 'beads on a string' structure. There are five main histone proteins involved in the structure of chromatin in eukaryotic cells. Histone H3 is the most extensively modified of the five histones. In the emerging field of epigenetics, it is thought that Histone H3 plays a key role, cause its sequence variants and variable modification states function in the dynamic and long term regulation of genes. |
| Conjucates | Unconjugated |
| Immunogen | A synthetic peptide: C-terminus of the human Histone H3. |
| Antigen Species | Human |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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