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Anti-Histone H3 Antibody (2C936)
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Catalog No. TMAY-00019
Anti-Histone H3 Antibody (2C936) is a Mouse antibody targeting Histone H3. Anti-Histone H3 Antibody (2C936) can be used in ICC/IF.
Anti-Histone H3 Antibody (2C936)
Anti-Histone H3 Antibody (2C936)
😃Good
Catalog No. TMAY-00019
Anti-Histone H3 Antibody (2C936) is a Mouse antibody targeting Histone H3. Anti-Histone H3 Antibody (2C936) can be used in ICC/IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 100 μL | $198 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Mouse Monoclonal Application: ICC/IF Reactivity: Human |
| Ig Type | Mouse IgG2b |
| Clone | 2C936 |
| Reactivity | Human |
| Verified Activity | Immunofluorescence staining of histone H3 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with mouse anti-human histone H3 monoclonal antibody (dilution ratio 1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-mouse IgG secondary antibody (green). Positive staining was localized to nucleus. |
| Application | ICC/IF |
| Recommended Dose | ICC-IF: 1:20-1:100 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Construction | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with a synthetic peptide corresponding to the C-terminus of the Human Histone H3. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | Histone H3 is a histone protein. Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. They play a central role in transcription regulation, DNA repair, DNA replication, and chromosomal stability. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. Histone H3 is characterized by a main globular domain and a long N-terminal tail. It is involved with the structure of the nucleosomes of the 'beads on a string' structure. There are five main histone proteins involved in the structure of chromatin in eukaryotic cells. Histone H3 is the most extensively modified of the five histones. In the emerging field of epigenetics, it is thought that Histone H3 plays a key role, cause its sequence variants and variable modification states function in the dynamic and long term regulation of genes. |
| Conjucates | Unconjugated |
| Immunogen | A synthetic peptide: C-terminus of the Human Histone H3 |
| Antigen Species | Human |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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