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Anti-Histone H3.3 Antibody (5A720)
😃Good
Catalog No. TMAC-01951
Alias MGC87783, MGC87782, Histone H3.B, Histone H3.A, Histone H3.3Q, H3f3b, H3F3A, H3F3, H33_HUMAN, H3.3B, H3.3A, H3.3, H3 histone family 3B, H3 histone family 3A
Anti-Histone H3.3 Antibody (5A720) is a Rabbit antibody targeting Histone H3.3. Anti-Histone H3.3 Antibody (5A720) can be used in WB,ICC/IF,IHC,ChIP.
Anti-Histone H3.3 Antibody (5A720)
Anti-Histone H3.3 Antibody (5A720)
😃Good
Catalog No. TMAC-01951Alias MGC87783, MGC87782, Histone H3.B, Histone H3.A, Histone H3.3Q, H3f3b, H3F3A, H3F3, H33_HUMAN, H3.3B, H3.3A, H3.3, H3 histone family 3B, H3 histone family 3A
Anti-Histone H3.3 Antibody (5A720) is a Rabbit antibody targeting Histone H3.3. Anti-Histone H3.3 Antibody (5A720) can be used in WB,ICC/IF,IHC,ChIP.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $297 | 7-10 days | |
| 100 μL | $496 | 7-10 days |
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| Description | Anti-Histone H3.3 Antibody (5A720) is a Rabbit antibody targeting Histone H3.3. Anti-Histone H3.3 Antibody (5A720) can be used in WB,ICC/IF,IHC,ChIP. |
| Synonyms | MGC87783, MGC87782, Histone H3.B, Histone H3.A, Histone H3.3Q, H3f3b, H3F3A, H3F3, H33_HUMAN, H3.3B, H3.3A, H3.3, H3 histone family 3B, H3 histone family 3A |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Western blot analysis of Histone H3.3 on BT-20 cell lysates using anti-Histone H3.3 antibody at 1/1,000 dilution. 2. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Histone H3.3 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Histone H3.3 antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Histone H3.3 antibody. Counter stained with hematoxylin. 5. Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti-Histone H3.3 antibody. Counter stained with hematoxylin. 6. ICC staining Histone H3.3 in BT-20 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. ICC staining Histone H3.3 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
| Application | |
| Recommended Dose | WB: 1:1000-2000; IHC: 1:50-200; ICC/IF: 1:50-200 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | Eukaryotic histones are basic and water soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fiber. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form the octamer; formed of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Over 80% of nucleosomes contain the linker Histone H1, derived from an intronless gene, that interacts with linker DNA between nucleosomes and mediates compaction into higher order chromatin. Histones are subject to posttranslational modification by enzymes primarily on their N-terminal tails, but also in their globular domains. Such modifications include methylation, citrullination, acetylation, phosphorylation, sumoylation, ubiquitination and ADP-ribosylation. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Uniprot ID |
| Molecular Weight | Theoretical: 15 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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