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Anti-Histone H3.3 Antibody (3Z104)

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Catalog No. TMAB-07132

Anti-Histone H3.3 Antibody (3Z104) is a Rabbit antibody targeting Histone H3.3. Anti-Histone H3.3 Antibody (3Z104) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF,ELISA.

Anti-Histone H3.3 Antibody (3Z104)

Anti-Histone H3.3 Antibody (3Z104)

😃Good
Catalog No. TMAB-07132
Anti-Histone H3.3 Antibody (3Z104) is a Rabbit antibody targeting Histone H3.3. Anti-Histone H3.3 Antibody (3Z104) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF,ELISA.
Pack SizePriceUSA WarehouseGlobal WarehouseQuantity
50 μL$2637-10 days7-10 days
100 μL$4717-10 days7-10 days
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction

Bioactivity
Description
Anti-Histone H3.3 Antibody (3Z104) is a Rabbit antibody targeting Histone H3.3. Anti-Histone H3.3 Antibody (3Z104) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF,ELISA.
Ig Type
IgG
Clone
3Z104
Reactivity
Human
Application
WB
IHC-P
IHC-Fr
ICC/IF
IF
ELISA
Recommended Dose
WB: 1:200-1000; IHC-P: 1:100-400; IHC-Fr: 1:100-400; ICC/IF: 1:100-500; IF: 1:100-500; ELISA: 1:5000-10000
Antibody Type
Monoclonal
Host SpeciesRabbit
Subcellular LocalizationNucleus. Chromosome.
ConstructionRecombinant Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundThe nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Interacts with HIRA, a chaperone required for its incorporation into nucleosomes. Interacts with ZMYND11; when trimethylated at 'Lys-36' (H3.3K36me3).
Antigen Details
Immunogen
Recombinant Protein: human Histone H3.3 protein, full length
Antigen Species
Human
Gene Name
H3-3A
Gene ID
3020
Protein Name
Histone H3.3
Uniprot ID
P84243
Function
Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Chemical Properties
Molecular WeightTheoretical: 15 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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