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Anti-Histone H2B Antibody (6C733)
Catalog No. TMAB-07090 Copy Product Info
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Anti-Histone H2B Antibody (6C733) is a Mouse antibody targeting Histone H2B. Anti-Histone H2B Antibody (6C733) can be used in IHC-P,IHC-Fr,IF.
Anti-Histone H2B Antibody (6C733)
Copy Product Info🥰Excellent
Catalog No. TMAB-07090
Anti-Histone H2B Antibody (6C733) is a Mouse antibody targeting Histone H2B. Anti-Histone H2B Antibody (6C733) can be used in IHC-P,IHC-Fr,IF.
Anti-Histone H2B Antibody
(6C733)
(6C733)
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| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 200 μg | $1,057 | 7-10 days | 7-10 days | |
| 50 μL | $222 | 7-10 days | 7-10 days | |
| 100 μL | $372 | 7-10 days | 7-10 days | |
| 200 μL | $529 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction
Bioactivity
Antigen Details
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| Description | Anti-Histone H2B Antibody (6C733) is a Mouse antibody targeting Histone H2B. Anti-Histone H2B Antibody (6C733) can be used in IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Clone | 6C733 |
| Reactivity | Mouse,Rat (predicted:Human) |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (Rat colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Histone H2B) Monoclonal Antibody, Unconjugated (TMAB-07090) at 1:800 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded (Mouse colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Histone H2B) Monoclonal Antibody, Unconjugated (TMAB-07090) at 1:800 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Histone H2B) Monoclonal Antibody, Unconjugated (TMAB-07090) at 1:800 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining. |
| Application | |
| Recommended Dose | IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Subcellular Localization | Nucleus. Chromosome. |
| Construction | Hybridoma Monoclonal Antibody |
| Purification | Protein G purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H2B family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Jul 2008]. |
| Immunogen | KLH conjugated synthetic peptide: human Histone H2B |
| Antigen Species | Human |
| Gene Name | H2BC3 |
| Gene ID | |
| Protein Name | Histone H2B type 1-B |
| Uniprot ID | |
| Function | Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. |
| Molecular Weight | Theoretical: 14 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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