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Anti-Histone H1.X Polyclonal Antibody
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Catalog No. TMAB-07062 Copy Product Info
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Anti-Histone H1.X Polyclonal Antibody is a Rabbit antibody targeting Histone H1.X. Anti-Histone H1.X Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-Histone H1.X Polyclonal Antibody
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 50 µL | $223 | 7-10 days | 7-10 days | |
| 100 µL | $371 | 7-10 days | 7-10 days | |
| 200 µL | $527 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
Stability & Storage
| Description | Anti-Histone H1.X Polyclonal Antibody is a Rabbit antibody targeting Histone H1.X. Anti-Histone H1.X Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Reactivity | Human,Mouse (predicted:Rat,Dog,Pig,Cow,Sheep) |
| Verified Activity | 1. Sample: Hela (Human) Cell Lysate at 30 μg Primary: Anti-Histone H1.X (TMAB-07062) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 22 kD Observed band size: 22 kD 2. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Histone H1.X) Polyclonal Antibody, Unconjugated (TMAB-07062) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining. |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | NuclearC. hromosome. |
| Tissue Specificity | Expressed ubiquitously. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene encodes a member of the histone H1 family. [provided by RefSeq, Jul 2008] |
| Immunogen | KLH conjugated synthetic peptide: human Histone H1.X |
| Antigen Species | Human |
| Gene Name | H1-10 |
| Gene ID | |
| Protein Name | Histone H1.10 |
| Uniprot ID | |
| Function | Histones H1 are necessary for the condensation of nucleosome chains into higher-order structures. |
| Molecular Weight | Theoretical: 22 kDa. Actual: 22 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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