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Anti-His Tag Monoclonal Antibody (1U977)

(Synonyms: HHHHHH tag, 6 His tag) Copy Product Info
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Synonyms: HHHHHH tag, 6 His tag

Catalog No. TMAT-1658 Copy Product Info
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Anti-His Tag Monoclonal Antibody (1U977) is a Mouse antibody targeting His Tag. Anti-His Tag Monoclonal Antibody (1U977) can be used in WB.

Anti-His Tag Monoclonal Antibody
(1U977)

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Product Introduction

Bioactivity
Description
Anti-His Tag Monoclonal Antibody (1U977) is a Mouse antibody targeting His Tag. Anti-His Tag Monoclonal Antibody (1U977) can be used in WB.
SynonymsHHHHHH tag, 6 His tag
Ig Type
IgG1
Clone
1U977
Reactivity
Tag
Application
Recommended Dose
WB: 1/500-1/1000
Antibody Type
Monoclonal
Host SpeciesMouse
ConstructionHybridoma Monoclonal Antibody
PurificationPurified by protein A or protein G
AppearanceLiquid
FormulationPBS with 0.05% sodium azide
Concentration1 mg/mL
Research BackgroundThe 6 X histidine motif is used as a tag on many recombinant proteins to facilitate purification.It can then be purified using immobilized metal ions,traditionally nickel,in a procedure termed immobilized-metal affinity chromatography.Further analysis of the protein can be achieved using an antibody specific to the 6 X histidine in techniques such as western blotting.The antibody recognizes the His-tag fused to targeted proteins in transfected or transformed cells.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Synthesized peptide of His Tag (AA: HHHHHHHH-KLH).
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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