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Anti-His Polyclonal Antibody
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Catalog No. TMAC-00038
Alias His Tag, HHHHHH, 6histag, 6*His
Anti-His Polyclonal Antibody is a Rabbit antibody targeting His. Anti-His Polyclonal Antibody can be used in WB.
Anti-His Polyclonal Antibody
Anti-His Polyclonal Antibody
😃Good
Catalog No. TMAC-00038Alias His Tag, HHHHHH, 6histag, 6*His
Anti-His Polyclonal Antibody is a Rabbit antibody targeting His. Anti-His Polyclonal Antibody can be used in WB.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $216 | 7-10 days | |
| 100 μL | $318 | 7-10 days |
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Chemical Properties
| Description | Anti-His Polyclonal Antibody is a Rabbit antibody targeting His. Anti-His Polyclonal Antibody can be used in WB. |
| Synonyms | His Tag, HHHHHH, 6histag, 6*His |
| Ig Type | IgG |
| Verified Activity | 1. Gel: 8%SDS-PAGE, Lysate: 2ug Recombinant 6-HIS Tagged protein, Primary antibody: 1/500 dilution, Secondary antibody dilution: 1/8000, Exposure time: 5 seconds.  |
| Application | |
| Recommended Dose | WB: 1:500-2000 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Construction | Polyclonal Antibody |
| Purification | Antigen affinity purification. |
| Appearance | Liquid |
| Formulation | Rabbit IgG in pH7.3 PBS, 0.05% NaN3, 50% Glycerol. |
| Research Background | The 6 X histidine motif is used as a tag on many recombinant proteins to facilitate purification.It can then be purified using immobilized metal ions,traditionally nickel,in a procedure termed immobilized-metal affinity chromatography.Further analysis of the protein can be achieved using an antibody specific to the 6 X histidine in techniques such as western blotting.The antibody recognizes the His-tag fused to the carboxy- termini of targeted proteins in transfected or transformed cells. |
| Conjucates | Unconjugated |
| Immunogen | KLH conjugated synthetic peptide: 18XHis |
| Molecular Weight | Actual: 46 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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