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Anti-His Polyclonal Antibody is a Rabbit antibody targeting His. Anti-His Polyclonal Antibody can be used in WB.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $216 | 7-10 days | |
100 μL | $318 | 7-10 days |
Description | Anti-His Polyclonal Antibody is a Rabbit antibody targeting His. Anti-His Polyclonal Antibody can be used in WB. |
Ig Type | IgG |
Application | |
Recommended Dose | WB: 1:500-2000 |
Antibody Type | Polyclonal |
Host Species | Rabbit |
Construction | Polyclonal Antibody |
Purification | Antigen affinity purification. |
Appearance | Liquid |
Formulation | Rabbit IgG in pH7.3 PBS, 0.05% NaN3, 50% Glycerol. |
Research Background | The 6 X histidine motif is used as a tag on many recombinant proteins to facilitate purification.It can then be purified using immobilized metal ions,traditionally nickel,in a procedure termed immobilized-metal affinity chromatography.Further analysis of the protein can be achieved using an antibody specific to the 6 X histidine in techniques such as western blotting.The antibody recognizes the His-tag fused to the carboxy- termini of targeted proteins in transfected or transformed cells. |
Conjucates | Unconjugated |
Immunogen | KLH conjugated synthetic peptide: 18XHis |
Antigen Species | KLH conjugated synthetic peptide: 18XHis |
Molecular Weight | Actual: 46 kDa. |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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