Anti-HIPK1 Polyclonal Antibody is a Rabbit antibody targeting HIPK1. Anti-HIPK1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human,Mouse,Rat (predicted:Dog,Pig,Cow,Horse,Rabbit,Sheep, Macaque Monkey)

1. Sample: Mcf-7 (Human) Cell Lysate at 40 μg
Primary: Anti-HIPK1 (TMAB-07045) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 131 kD
Observed band size: 131 kD
2. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (HIPK1) Polyclonal Antibody, Unconjugated (TMAB-07045) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
3. Sample:
Lane 1: Mouse Testis tissue lysates
Lane 2: Rat Testis tissue lysates
Lane 3: Human HeLa cell lysates
Lane 4: Human 293T cell lysates
Primary: Anti-HIPK1 (TMAB-07045) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 131 kDa
Observed band size: 130 kDa

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human HIPK1

Human

HIPK1

Homeodomain-interacting protein kinase 1

May play a role as a corepressor for homeodomain transcription factors. Phosphorylates DAXX in response to stress, and mediates its translocation from the nucleus to the cytoplasm. May be involved in malignant squamous cell tumor formation.