Anti-HIF1AN Antibody (1I6) is a Mouse antibody targeting HIF1AN. Anti-HIF1AN Antibody (1I6) can be used in WB,ICC/IF.

IgG1, k

1I6

Human

1. Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (HIF1AN) monoclonal Antibody, Unconjugated (TMAB-07031) 1:50, 90 minutes at 37°C; followed by a conjugated Goat Anti-Mouse IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.Dylight® 554 Phalloidin (red) was used to stain the cell Cytoplasmic actin.
2. Sample:
Lane 1: 293 cell lysates
Lane 2: Jurkat cell lysates
Lane 3: ZR-75-1 cell lysates
Primary: Anti-HIF1AN (TMAB-07031) at 1/4000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 38 kD
Observed band size: 38 kD

WB: 1:1000-5000; ICC/IF: 1:50-200

Monoclonal

KLH conjugated synthetic peptide: human HIF1AN

Human

HIF1AN

Hypoxia-inducible factor 1-alpha inhibitor

Hydroxylates HIF-1 alpha at 'Asp-803' in the C-terminaltransactivation domain (CAD). Functions as an oxygen sensor and,under normoxic conditions, the hydroxylation prevents interactionof HIF-1 with transcriptional coactivators includingCbp/p300-interacting transactivator. Involved in transcriptionalrepression through interaction with HIF1A, VHL and histonedeacetylases. Hydroxylates specific Asn residues within ankyrinrepeat domains (ARD) of NFKB1, NFKBIA, NOTCH1, ASB4, PPP1R12A andseveral other ARD-containing proteins. Also hydroxylates Asp andHis residues within ARDs of ANK1 and TNKS2, respectively.Negatively regulates NOTCH1 activity, accelerating myogenicdifferentiation. Positively regulates ASB4 activity, promotingvascular differentiation.