Anti-HIF1A Polyclonal Antibody is a Rabbit antibody targeting HIF1A. Anti-HIF1A Polyclonal Antibody can be used in ICC/IF.

IgG

Human

1. 4% Paraformaldehyde-fixed Hela (treated with 500 uM CoCl2 for 6 hours) (H) cell; Triton X-100 at r. T. for 20 min; Antibody incubation with (HIF-1 Alpha) polyclonal Antibody, unconjugated (TMAB-07030) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green) at 37°C for 90 min, DAPI (blue) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
2. 4% Paraformaldehyde-fixed HepG2 (treated with 500 uM CoCl2 for 6 hours) (H) cell; Triton X-100 at r. T. for 20 min; Antibody incubation with (HIF-1 Alpha) polyclonal Antibody, unconjugated (TMAB-07030) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green) at 37°C for 90 min, DAPI (blue) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.

ICC/IF: 1:50-200

Polyclonal

Recombinant Protein: human HIF-1 Alpha protein

Human

HIF1A

Hypoxia-inducible factor 1-alpha

Functions as a master transcriptional regulator of theadaptive response to hypoxia. Under hypoxic conditions activatesthe transcription of over 40 genes, including, erythropoietin,glucose transporters, glycolytic enzymes, vascular endothelialgrowth factor, and other genes whose protein products increaseoxygen delivery or facilitate metabolic adaptation to hypoxia.Plays an essential role in embryonic vascularization, tumorangiogenesis and pathophysiology of ischemic disease. Binds to coreDNA sequence 5'-[AG]CGTG-3' within the hypoxia response element(HRE) of target gene promoters. Activation requires recruitment oftranscriptional coactivators such as CREBPB and EP300. Activity isenhanced by interaction with both, NCOA1 or NCOA2. Interaction withredox regulatory protein APEX seems to activate CTAD andpotentiates activation by NCOA1 and CREBBP.