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Anti-HIF-2 alpha Polyclonal Antibody
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Catalog No. TMAB-00859
Anti-HIF-2 alpha Polyclonal Antibody is a Rabbit antibody targeting HIF-2 alpha. Anti-HIF-2 alpha Polyclonal Antibody can be used in WB,ICC/IF,FCM.
Anti-HIF-2 alpha Polyclonal Antibody
Anti-HIF-2 alpha Polyclonal Antibody
😃Good
Catalog No. TMAB-00859
Anti-HIF-2 alpha Polyclonal Antibody is a Rabbit antibody targeting HIF-2 alpha. Anti-HIF-2 alpha Polyclonal Antibody can be used in WB,ICC/IF,FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $221 | 7-10 days | |
| 100 μL | $372 | 7-10 days | |
| 200 μL | $527 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Antibody Type: Rabbit Polyclonal Application: WB,ICC/IF,FCM Reactivity: Mouse,Rat (predicted:Human) |
| Ig Type | IgG |
| Reactivity | Mouse,Rat (predicted:Human) |
| Verified Activity | 25 μg total protein per Lane of various lysates probed with HIF 2 alpha polyclonal antibody, unconjugated (TMAB-00859) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at RT for 60 min. |
| Application | WB,ICC/IF,FCM |
| Recommended Dose | WB: 1:500-2000; ICC/IF: 1:50-200; FCM: 1:20-100 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Nucleus (Potential). |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | Transcription factor involved in the induction of oxygen regulated genes. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung. May also play a role in the formation of the endothelium that gives rise to the blood brain barrier. Potent activator of the Tie-2 tyrosine kinase expression. Activation requires recruitment of transcriptional coactivators such as CREBPB and probably EP300. Interaction with redox regulatory protein APEX seems to activate CTAD. |
| Immunogen | KLH conjugated synthetic peptide: mouse HIF 2 Alpha |
| Antigen Species | Mouse |
| Gene ID | 13819 |
| Uniprot ID | |
| Biology Area | Response to hypoxia,HIF,HIF,HIF,HLH,Mitochondrial transcription,Hypoxia |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
Sci Citations
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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