Shopping Cart
- Remove All
Your shopping cart is currently empty
Anti-HIF-1 alpha Polyclonal Antibody 3
😃Good
Catalog No. TMAB-00857
Alias PASD8, MOP1, hypoxia inducible factor 1, α subunit (basic helix-loop-helix transcription factor), hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor), HIF1-α, HIF-1α, HIF1-ALPHA, HIF-1alpha, HIF-1A, HIF-1 α, HIF1, bHLHe78
Anti-HIF-1 alpha Polyclonal Antibody 3 is a Rabbit antibody targeting HIF-1 alpha. Anti-HIF-1 alpha Polyclonal Antibody 3 can be used in WB,FCM.
Anti-HIF-1 alpha Polyclonal Antibody 3
Anti-HIF-1 alpha Polyclonal Antibody 3
😃Good
Catalog No. TMAB-00857Alias PASD8, MOP1, hypoxia inducible factor 1, α subunit (basic helix-loop-helix transcription factor), hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor), HIF1-α, HIF-1α, HIF1-ALPHA, HIF-1alpha, HIF-1A, HIF-1 α, HIF1, bHLHe78
Anti-HIF-1 alpha Polyclonal Antibody 3 is a Rabbit antibody targeting HIF-1 alpha. Anti-HIF-1 alpha Polyclonal Antibody 3 can be used in WB,FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $221 | 7-10 days | |
| 100 μL | $374 | 7-10 days | |
| 200 μL | $527 | 7-10 days |
Add to Cart
Add to Quotation
Bulk & Custom
Questions
View MoreContact us for more batch information
Resource Download
Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-HIF-1 alpha Polyclonal Antibody 3 is a Rabbit antibody targeting HIF-1 alpha. Anti-HIF-1 alpha Polyclonal Antibody 3 can be used in WB,FCM. |
| Alias | PASD8, MOP1, hypoxia inducible factor 1, α subunit (basic helix-loop-helix transcription factor), hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor), HIF1-α, HIF-1α, HIF1-ALPHA, HIF-1alpha, HIF-1A, HIF-1 α, HIF1, bHLHe78 |
| Ig Type | IgG |
| Reactivity | Human (predicted:Mouse,Rat,Chicken) |
| Verified Activity | 1. Sample: Lane 1: Hela (Human) Cell Lysate at 30 μg Lane 2: A431 (Human) Cell Lysate at 30 μg Lane 3: U251 (Human) Cell Lysate at 30 μg Lane 4: HepG2 (Human) Cell Lysate at 30 μg Primary: Anti-HIF-1 Alpha (TMAB-00857) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 92 kDa Observed band size: 120 kDa 2. Blank control (blue line): Hela (fixed with 80% methanol (5 min at-20°C) and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature). Primary Antibody (green line): Rabbit Anti-HIF-1 Alpha antibody (TMAB-00857), Dilution: 1 μg/10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC, Dilution: 1 μg/test. 3. Sample: Lane 1: Recombinant human HIF-1 Alpha protein, N-Trx-His Primary: Anti-HIF-1 Alpha (TMAB-00857) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 92 kDa Observed band size: 52 kDa 4. The HepG2 (H) （treated with 500uM CoCl2 for 6 hours) cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at-20°C,the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at room temperature). Primary Antibody (green): Rabbit Anti-HIF-1 Alpha antibody (TMAB-00857): 1 μg/10^6 cells; Secondary Antibody (white blue): Goat anti-Rabbit IgG-FITC: 1 μg/test. Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. |
| Application | WB,FCM |
| Recommended Dose | WB: 1:500-2000; FCM: 1μg/Test |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cytoplasm. Nucleus. |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | Preservative: 0.02% Proclin300, Constituents: 1% BSA, 0.01M PBS, pH7.4. |
| Research Background | Hypoxia inducible factor alpha not only plays a particularly important role in maintaining normal physiological functions of the body under hypoxic conditions, but also plays a crucial role in pathological processes such as tumor growth and neuronal apoptosis HIF1 alpha can regulate the expression levels of many downstream genes Mammalian cells exhibit HIF under low oxygen pressure conditions. HIF is a transcription factor that stabilizes cellular hypoxia. |
| Immunogen | KLH conjugated synthetic peptide: human HIF-1 Alpha |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID | |
| Biology Area | HIF,Hydroxylases,HIF,Jumonji containing proteins,Methylation |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
Calculator
For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
Sci Citations
Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
Keywords
Preview
Related Tags: buy Anti-HIF-1 alpha Polyclonal Antibody 3 | purchase Anti-HIF-1 alpha Polyclonal Antibody 3 | Anti-HIF-1 alpha Polyclonal Antibody 3 cost | order Anti-HIF-1 alpha Polyclonal Antibody 3
- TOP
Feedback
Hello! How can I help you today? 
Copyright © 2015-2025 TargetMol Chemicals Inc. All Rights Reserved.