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Anti-HIF-1 alpha Polyclonal Antibody 2
😃Good
Catalog No. TMAB-00856
Anti-HIF-1 alpha Polyclonal Antibody 2 is a Rabbit antibody targeting HIF-1 alpha. Anti-HIF-1 alpha Polyclonal Antibody 2 can be used in WB,ICC/IF,FCM.
Anti-HIF-1 alpha Polyclonal Antibody 2
Anti-HIF-1 alpha Polyclonal Antibody 2
😃Good
Catalog No. TMAB-00856
Anti-HIF-1 alpha Polyclonal Antibody 2 is a Rabbit antibody targeting HIF-1 alpha. Anti-HIF-1 alpha Polyclonal Antibody 2 can be used in WB,ICC/IF,FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $220 | 7-10 days | |
| 100 μL | $372 | 7-10 days | |
| 200 μL | $528 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Antibody Type: Rabbit Polyclonal Application: WB,ICC/IF,FCM Reactivity: Human (predicted:Mouse,Rat,Dog,Pig,Cow,Horse,Rabbit,Sheep) |
| Ig Type | IgG |
| Reactivity | Human (predicted:Mouse,Rat,Dog,Pig,Cow,Horse,Rabbit,Sheep) |
| Verified Activity | 1. Blank control (blue line): Hela (fixed with 80% methanol (5 min) and and then permeabilized with 0.01M PBS-Tween for 20 min) Primary Antibody (green line): Rabbit Anti-HIF-1 Alpha antibody (TMAB-00856), Dilution: 1 μg/10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC, Dilution: 1 μg/test. 2. Sample: Lane 1: Recombinant human HIF-1 Alpha protein, N-Trx-His at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 92 kDa Observed band size: 47 kDa |
| Application | WB,ICC/IF,FCM |
| Recommended Dose | WB: 1:500-2000; ICC/IF: 1:50-200; FCM: 1μg/Test |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cytoplasm. Nucleus. |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | Preservative: 0.02% Proclin300, Constituents: 1% BSA, 0.01M PBS, pH7.4. |
| Research Background | Hypoxia inducible factor alpha not only plays a particularly important role in maintaining normal physiological functions of the body under hypoxic conditions, but also plays a crucial role in pathological processes such as tumor growth and neuronal apoptosis HIF1 alpha can regulate the expression levels of many downstream genes Mammalian cells exhibit HIF under low oxygen pressure conditions. HIF is a transcription factor that stabilizes cellular hypoxia. |
| Immunogen | KLH conjugated synthetic peptide: human HIF-1 Alpha |
| Antigen Species | Human |
| Gene ID | 3091 |
| Uniprot ID | |
| Biology Area | HIF,Hydroxylases,HIF,Jumonji containing proteins,Methylation |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
Sci Citations
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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