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Anti-HIF-1 Alpha Antibody (9V220)
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Catalog No. TMAB-07027
Anti-HIF-1 Alpha Antibody (9V220) is a Rabbit antibody targeting HIF-1 Alpha. Anti-HIF-1 Alpha Antibody (9V220) can be used in WB,ICC/IF,FCM.
Anti-HIF-1 Alpha Antibody (9V220)
Anti-HIF-1 Alpha Antibody (9V220)
😃Good
Catalog No. TMAB-07027
Anti-HIF-1 Alpha Antibody (9V220) is a Rabbit antibody targeting HIF-1 Alpha. Anti-HIF-1 Alpha Antibody (9V220) can be used in WB,ICC/IF,FCM.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 25 μL | $150 | 7-10 days | 7-10 days | |
| 50 μL | $261 | 7-10 days | 7-10 days | |
| 100 μL | $473 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-HIF-1 Alpha Antibody (9V220) is a Rabbit antibody targeting HIF-1 Alpha. Anti-HIF-1 Alpha Antibody (9V220) can be used in WB,ICC/IF,FCM. |
| Ig Type | IgG |
| Clone | 9V220 |
| Reactivity | Human (predicted:Mouse,Rat) |
| Application | |
| Recommended Dose | WB: 1:500-2000; ICC/IF: 1:50-200; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cytoplasm. Nucleus. |
| Tissue Specificity | Expressed in most tissues with highest levels in kidney and heart. Overexpressed in the majority of common human cancers and their metastases, due to the presence of intratumoral hypoxia and as a result of mutations in genes encoding oncoproteins and tumor suppressors. |
| Construction | Recombinant Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 10mM phosphate buffered saline (pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| Research Background | Functions as a master transcriptional regulator of the adaptive response to hypoxia.Under hypoxic conditions, activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. |
| Immunogen | A synthesized peptide: human HIF 1 alpha |
| Antigen Species | Human |
| Gene Name | HIF1A |
| Gene ID | |
| Protein Name | Hypoxia-inducible factor 1-alpha |
| Uniprot ID | |
| Function | Functions as a master transcriptional regulator of theadaptive response to hypoxia. Under hypoxic conditions activatesthe transcription of over 40 genes, including, erythropoietin,glucose transporters, glycolytic enzymes, vascular endothelialgrowth factor, and other genes whose protein products increaseoxygen delivery or facilitate metabolic adaptation to hypoxia.Plays an essential role in embryonic vascularization, tumorangiogenesis and pathophysiology of ischemic disease. Binds to coreDNA sequence 5'-[AG]CGTG-3' within the hypoxia response element(HRE) of target gene promoters. Activation requires recruitment oftranscriptional coactivators such as CREBPB and EP300. Activity isenhanced by interaction with both, NCOA1 or NCOA2. Interaction withredox regulatory protein APEX seems to activate CTAD andpotentiates activation by NCOA1 and CREBBP. |
| Molecular Weight | Theoretical: 93 kDa. Actual: 120 kDa. |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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