Anti-HIF-1 Alpha Antibody (9V220) is a Rabbit antibody targeting HIF-1 Alpha. Anti-HIF-1 Alpha Antibody (9V220) can be used in WB,ICC/IF,FCM.

IgG

9V220

Human (predicted:Mouse,Rat)

1. 4% Paraformaldehyde-fixed Hela (H) (HeLa treated with 500 uM CoCl2 for 6 hours) Cell; Triton X-100 at r. T. for 20 min; Antibody incubation with (HIF-1 Alpha) monoclonal Antibody, unconjugated (TMAB-07027) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green) at 37°C for 90 min, DAPI (blue) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
2. 4% Paraformaldehyde-fixed HepG2 (H) (HepG2 treated with 500 uM CoCl2 for 6 hours) Cell; Triton X-100 at r. T. for 20 min; Antibody incubation with (HIF-1 Alpha) monoclonal Antibody, unconjugated (TMAB-07027) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green) at 37°C for 90 min, DAPI (blue) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
3. Hep-G2 (H) cells were treated with or without CoCl2 (500 uM) for 6 h, 25 μg total protein per lane of cell lysates (see on figure) probed with HIF-1 Alpha monoclonal antibody, unconjugated (TMAB-07027) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
4. The Hela (treated with 500 uM CoCl2 for 6 hours) (H) cells were fixed with 4% PFA (10 min at r. T.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.). Primary Antibody (green): Rabbit Anti-HIF-1 Alpha antibody (TMAB-07027): 1:50-100/10^6 cells; Secondary Antibody (white blue): Goat anti-Rabbit IgG-BF488: 1 μg/test. Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed.

WB: 1:500-2000; ICC/IF: 1:50-200; FCM: 1:50-100

Monoclonal

A synthesized peptide: human HIF 1 alpha

Human

HIF1A

Hypoxia-inducible factor 1-alpha

Functions as a master transcriptional regulator of theadaptive response to hypoxia. Under hypoxic conditions activatesthe transcription of over 40 genes, including, erythropoietin,glucose transporters, glycolytic enzymes, vascular endothelialgrowth factor, and other genes whose protein products increaseoxygen delivery or facilitate metabolic adaptation to hypoxia.Plays an essential role in embryonic vascularization, tumorangiogenesis and pathophysiology of ischemic disease. Binds to coreDNA sequence 5'-[AG]CGTG-3' within the hypoxia response element(HRE) of target gene promoters. Activation requires recruitment oftranscriptional coactivators such as CREBPB and EP300. Activity isenhanced by interaction with both, NCOA1 or NCOA2. Interaction withredox regulatory protein APEX seems to activate CTAD andpotentiates activation by NCOA1 and CREBBP.